由ABO正反定型不符发现类孟买血型

The discovery of para-bombay phenotype by ABO forward and reverse typing unconformity

目的:研究类孟买血型的鉴定。方法:用血型血清学、吸收放散试验检测ABH抗原及相应抗体,并进行唾液血型物质检测以及血型基因分型最终鉴定类孟买血型。结果:常规血型血清学未检出B、H抗原、吸收放散试验检出H抗原,利用新生儿红细胞和标准红细胞与患者血清反应结果的对比检出抗H或抗HI,Lewis血型为Le(a-b+),唾液中检出B、H血型物质从而鉴定该患者血型为Bmh,和该例患者血型基因分型结果一致。结论:规范疑难血型的鉴定是发现稀有血型和保证安全输血的必要条件。

Objective：To study the identification of para-Bombay phenotype.Method：The ABH antigens and corresponding antibodies were detected by blood group serology as well as absorption and dispersion test.The para-Bombay phenotype was finally identified by carrying out saliva blood group material detection and blood type genotyping.Result：B and H antigens were not detected in conventional blood genegroup serology,and H antigen was detected by absorption and dispersion test.Anti H or HI were detected by comparing the result of neonatal red blood cells responding with patient serum and that of standard red blood cells responding with patient serum.Lewis＇ blood type is Le（a-b＋）,and B and H blood type material were detected in his saliva so that the blood type of the patient was identified as Bmh,the same as the blood type genotyping result of the patient.Conclusion：It is necessary to regulate the identification of difficult blood types for finding rare blood types and ensuring safe blood transfusion.