侵染牡丹的烟草脆裂病毒的检测鉴定及序列分析

Molecular detection, identification and genetic diversity of Tobacco rattle virus on peony

为明确江苏省牡丹上烟草脆裂病毒(Tobacco rattle virus,TRV)的发生情况,利用ELISA和RT-PCR方法对采集自扬州市的40份牡丹叶片样品进行了检测鉴定,测定了其中一个分离物Peony-11中TRV RNA1分子的部分蛋白编码区序列,并结合Gen Bank中已报道的相关序列对其进行了多样性和系统发生分析。结果显示,江苏省扬州市牡丹上TRV的检出率高达62.5%;序列分析表明本研究获得的TRV牡丹分离物Peony-11与Gen Bank中其它63个分离物的核苷酸序列一致率为90.5%~99.7%;系统发生和遗传距离分析表明TRV可以分成2个组10个亚组,组间、亚组间具有较为清晰的地理和寄主特异性,其中Peony-11分离物位于亚组I-1。

In order to investigate the occurrence of Tobacco rattle virus（TRV） in peony in Jiangsu Province, 40 leaf samples collected from Yangzhou were detected by ELISA and RT-PCR. Partial ORF1 coding region of TRV RNA1（Peony-11） was sequenced and analyzed with related sequences on Gen Bank.According to the results, the detection rate of TRV in peony was 62.5% in Yangzhou. Sequence analysis indicated that the TRV peony isolate determined in the present study shared nucleotide identities of90.5%-99.7% with 63 TRV isolates downloaded from Gen Bank. Phylogenetic and genetic distance analysis demonstrated that TRVs were clustered into two groups, ten subgroups with clear geography and host specificity, while the TRV Peony-11 isolate determined here belonged to subgroup I-1.