心脑舒通及其单体成分对小鼠脑微血管内皮细胞缺氧复氧炎症损伤的影响

The anti-inflammatory effect of Xinnaoshutong in hypoxia and re oxygenation mouse brain microvascular endothelial cells

目的探讨心脑舒通(XNST)及其单体成分对小鼠脑微血管内皮细胞株b End.3缺氧复氧损伤后炎症相关因子TNF-α、IL-6、IL-1β的影响。方法通过建立b End.3细胞缺氧复氧损伤模型,检测不同浓度心脑舒通(0.1、1.0、10.0、50.0μg/ml)或单体成分(10.0μmol/ml)对b End.3细胞活力的影响,用酶联免疫吸附试验(ELISA)定量检测炎症相关因子肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-1β的表达。结果心脑舒通对b End.3细胞胞质TNF-α的合成具有明显抑制作用,对细胞上清IL-6的分泌量有抑制作用但差异无统计学意义(P>0.05),而在b End.3细胞中IL-1β的表达不明显。结论心脑舒通通过抑制炎症因子TNF-α的合成,可能是其发挥治疗脑缺血炎症损伤的途径之一。

Objective To investigate the effect of Xinnaoshutong and its monomer component on the inflammatory cytokines TNF-α,IL-6,IL-1β in cerebral ischemia and reperfusion mouse brain microvascular endothelial cells line b End. 3. Methods Ischemia-reperfusion inflammation injury b End.3 cells model was established,the effect of different concentrations Xinnaoshutong（ 0.1,1.0,10.0,50.0 μg/ml） or the monomer component（ 10μmol/ml） on cells vitality of b End. 3 was detected. Inflammation-related factor TNF-α,IL-6,IL-1βlevel were measured by ELISA.Results Xinnaoshutong significantly inhibited b End. 3 cell cytoplasmic synthesis of TNF-α,while the supernatants of IL-6 secretion was inhibited but there was no statistical difference. The cytoplasmic synthesis or supernatants of IL-1β expression in b End.3 cells was not obvious.Conclusions Xinnaoshutong plays a therapeutic role in inflammatory injury of cerebral ischemia by inhibiting the synthesis inflammatory cytokines of TNF-α.