量子点免疫荧光成像技术同步检测乳腺癌黏蛋白1及Ⅳ型胶原蛋白的临床意义

Clinical significances of the synchronous detection of mucin 1 and collagen Ⅳ in breast cancer by using quantum dots immunofluorescence imaging technique

目的利用量子点免疫荧光成像技术，探讨黏蛋白1及Ⅳ型胶原蛋白与浸润性乳腺癌临床病理特征的关系。方法应用量子点免疫荧光成像技术和免疫组织化学技术检测2007年7月至2010年7月桂林医学院附属医院收治的94例乳腺癌组织中黏蛋白1及Ⅳ型胶原蛋白的表达，分析黏蛋白1及Ⅳ型胶原蛋白定量参数与乳腺癌患者临床病理特征的相关性及其与预后的关系。结果量子点免疫荧光成像技术和免疫组织化学技术标记人乳腺癌组织中黏蛋白1阳性率分别为73.4 %（69/94）、69.1 %（65/94），Ⅳ型胶原蛋白阳性率分别为53.2 %（50/94）、47.9 %（45/94），差异均无统计学意义（均P〉0.05），两种方法标记的效果具有一致性（κ=0.763，P=0.000；κ=0.759，P=0.000）。分析量子点免疫荧光成像技术检测结果，黏蛋白1与Ⅳ型胶原蛋白的表达呈负相关（r=-0.883，P〈0.01）；黏蛋白1和Ⅳ型胶原蛋白的表达与肿瘤大小（F=3.683，P=0.029；F=4.922，P=0.009）、组织学分级（F=3.529，P=0.033；F=3.912，P=0.023）、淋巴结转移（t=-4.868，P=0.000；t=3.868，P=0.000）、病理分期（t=-8.337，P=0.000；t=5.962，P=0.000）和5年无病生存率（均P=0.000）均有相关性。结论利用量子点免疫荧光成像技术可同时检测黏蛋白1及Ⅳ型胶原蛋白，可为乳腺癌生物学行为及预后判断提供依据。

ObjectiveTo investigate the relationship between the expression of mucin 1 and collagen Ⅳ and the clinical characteristics in invasive breast cancer by using the quantum dots immunofluorescence imaging technique.MethodsThe expressions of mucin 1 and collagen Ⅳ were detected simultaneously by quantum dots immunofluorescence imaging technique in 94 cases of breast cancer from July 2007 to July 2010 in Affiliated Hospital of Guilin Medical University. The correlation of mucin 1 and collagen Ⅳ quantitative parameters with clinicopathological features and the prognosis were also analyzed.ResultsThe positive rates of mucin 1 in human breast cancer tissues marked by quantum dots immunofluorescence imaging technique and immunohistochemistry were 73.4 % （69/94） and 69.1 % （65/94）, the positive rates of collagen Ⅳ were 53.2 % （50/94） and 47.9 % （45/94）, and the differences were not statistically significant （both P 〉 0.05） Quantum dots immunofluorescence imaging technique was consistent with conventional immunohistochemistry （IHC） in detecting the expressions of mucin 1 and collagen Ⅳ in human breast cancer tissues （κ = 0.763, P = 0.000; κ=0.759, P = 0.000）. The expression of mucin 1 was negatively correlated with collagen Ⅳ （r = -0.883, P 〈 0.01）. The expressions of mucin 1 and collagen Ⅳ were respectively associated with tumor size （F = 3.683, P = 0.029; F = 4.922, P = 0.009）, histological grading （F = 3.529, P = 0.033; F = 3.912, P = 0.023）, lymph node metastasis （t = -4.868, P = 0.000; t = 3.868, P = 0.000）, pathological stage （t = -8.337, P = 0.000; t = 5.962, P = 0.000） and 5-year disease free survival rate （both P = 0.000）, and the differences were statistically significant （all P 〈 0.05）.ConclusionThe co-detection of mucin 1 and collagen Ⅳ by using quantum dots immunofluorescence imaging technique provides direct evidence determining the biologic behaviors of breast cancer and evaluating the prognosis.