人KIAA0146基因及蛋白质的生物信息学分析

Bioinformatics Analysis of Human KIAA0146 Gene and Protein

KIAA0146是同源重组修复蛋白质复合体BLM/RAD51的支架蛋白质。当前KIAA0146相关的实验研究非常少。本文采用生物信息学方法对KIAA0146基因及其蛋白质进行快速分析以期为其往后的实验研究提供线索。首先发现KIAA0146基因存在多个转录起始位点及启动子,且启动子(2 127~2 177 bp)和(3 127~3 177 bp)的侧翼各有2个Cp G岛。然后发现KIAA0146是亲水的不稳定蛋白质。由于未发现KIAA0146蛋白包含信号肽和跨膜区域,所以它可能既不是分泌蛋白质也不是跨膜蛋白质。但是KIAA0146可能是细胞核蛋白质,在其N端包含核定位信号"RARGSKRKR",而且构建的KIAA0146蛋白的三级结构提示它可能具有核酸结合结构域。最后发现RAD51、ATM、RAD51B、MRE11A、BLM、DNA2、RMI1、BRCA1、BRCA2和XRCC2可能与KIAA0146相互作用,而且KIAA0146可能通过与这10个蛋白质相互作用参与DNA损伤应答及修复、发育和细胞代谢调节等生物过程。总之,文中对KIAA0146基因结构及其蛋白质的亚细胞定位、三级结构和潜在的分子功能等进行了预测分析,为往后实验研究KIAA0146提供了重要的理论依据和新线索。

KIAA0146 is a scaffold protein of homologous recombinant protein complex BLM/RAD51. Current-ly, there are few KIAA0146-related experimental studies. The effective bioinformatics analyses were therefore performed for providing clues for further experimental study of KIAA0146. Several transcription start sites and promoters were found at the KIAA0146 gene. Besides, two Cp G islands were found on the flank of promoter（2 127-2 177 bp） and（3 127-3 177 bp）, respectively. It was found that KIAA0146 was an unstable hydrophilic protein without signal peptide and transmembrane domain. It was therefore considered as neither a secreted protein nor a transmembrane protein. However, the KIAA0146 was considered as nuclear protein with nuclear localization signal＂RARGSKRKR＂at its N-terminal. The constructed 3 D structure of KIAA0146 showed that it contained a nucleic acid-binding domain. It was finally found that RAD51, ATM, RAD51 B, MRE11 A, BLM,DNA2, RMI1, BRCA1, BRCA2 and XRCC2 might interact with KIAA0146. In addition, KIAA0146 was con-sidered to involve into the biological processes including DNA damage response and repair, development and cell metabolism regulation through these interacting proteins. In conclusion, the analyses of gene structure,protein subcellular location, 3 D structure and potential molecular function of KIAA0146 provide important theories and new clues for further experimental study on this molecule.