肝郁与精瘀的实验研究

The Experimental Study on the Correlation Between Liver Depression and Sperm Stasis

目的:通过建立雄性大鼠精瘀动物模型,探讨肝郁与精瘀的相关性。方法:将30只雄性大鼠随机分为对照组,肝郁药物组,肝郁精瘀组,每组各10只,采用慢性束缚应激方法建立肝郁大鼠模型,在肝郁病理基础上,继续予以束缚处理以建立精瘀模型,并通过检测血清雌二醇(estradiol,E2)、酸性磷酸酶(acidphosphatase,ACP)、α-糖苷酶(α-glycosidase,α-GLU)等指标的改变以确定肝郁致精瘀模型的建立。按每毫升含3 g生药给肝郁药物组大鼠灌胃柴胡疏肝散,其他两组则灌胃等体积的蒸馏水,连续4周。在末次刺激的24 h后,对三组大鼠进行行为学观察,随即股动脉取血,静置后离心,取血清待测。采用ELASA方法检测E2、ACP、α-GLU的浓度。结果:肝郁模型组、肝郁精瘀组与对照组大鼠比较,行为多表现为倦怠少动、叫声尖细、反应迟缓、饮食减少、毛发晦暗、大便松散甚呈水样;三组E2浓度比较有差异,但无统计学意义(P>0.05);肝郁药物组与肝郁精瘀组比较,ACP明显降低(P<0.05);肝郁精瘀组与肝郁药物组比较,α-GLU明显升高(P<0.05)。结论:柴胡疏肝散可改变精瘀大鼠行为,改善精瘀大鼠相关实验室指标,提示情志-肝郁-精瘀有一定相关性。

Objective： By establishing the animal model of sperm stasis in male rats,the correlation between liver depression and sperm stasis is explored. Methods： Thirty male rats were randomly divided into the control group,the liver depression drug group and the liver depression-sperm stasis group,with 10 rats in each group. The rat model of liver depression was established by chronic restraint stress. On the basis of liver depression pathology,the model of sperm stasis was established by continuing to be shackled,and the model of sperm stasis induced by liver depression was established by detecting the changes of estradiol（ E2）acid phosphatase（ ACP）,α-glycosidase,α-GLU）. The rats in the liver depression group were given Bupleurum Liver-Soothing Powder according to 3 g of raw medicine per milliliter and the other two groups were given distilled water of the same volume. All the groups were treated for 4 weeks. 24 hours after the last stimulation,the behaviors of the three groups of rats were observed,then the femoral artery blood was collected,centrifuged and serum was collected. Using ELASA,concentrations of E2; ACP; α-GLU were measured. Results： Compared with the control group,in the liver depression drug group,and the liver depressionsperm stasis group,most of the behaviors of rats were manifested as burnout,less movement,shrill,slow response,reduced diet,dark hair,loose stool and watery stool. The three groups＇ E2 concentration was different,but there was no statistical significance（ P〉 0. 05）. Compared with the liver depression-sperm stasis group,ACP of the liver depression drug group was significantly lower（ P〉 0. 05）,whileα-GLU of the liver depression-sperm stasis group was significantly higher（ P 〈0. 05）. Conclusion： Bupleurum Liver-Soothing Powder can change the behavior of rats with sperm stasis,improve the related laboratory indexes of rats,which suggests that there is a certain correlation between emotions and liver depression and sperm stasis.