CARMA3在胆管癌细胞中的表达及功能

CARMA3 expression in cholangiocarcinoma cells and its function

目的:探讨接头蛋白CARMA3在胆管癌细胞中的表达及功能。方法:用qRT-PCR技术检测胆管癌HUCCT1细胞与RBE细胞以及正常胆管细胞HIBEC中CARMA3的mRNA表达。用siRNA技术沉默HUCCT1细胞与RBE细胞中CARMA3的表达后,分别通过CCK-8法、流式细胞术、Transwell实验检测细胞增殖、凋亡、细胞周期以及转移和侵袭能力的变化。结果:两种胆管癌细胞中CARMA3的mRNA表达水平均明显高于正常胆管细胞HIBEC(HUCCT1 vs.HIBEC:t=5.321,P=0.011;RBE vs.HIBEC:t=5.932,P=0.008)。沉默CARMA3的表达后,两种胆管癌细胞的增殖、转移和细胞侵袭能力被明显抑制,G_1期阻滞明显增加,细胞凋亡率明显升高(均P<0.05)。结论:CARMA3在胆管癌细胞中表达上调,其作用可能与促进细胞增殖、侵袭、转移并抑制细胞凋亡。

Objective： To investigate the expression of the adaptor protein CARMA3 in cholangiocarcinoma cells and its function.Methods： The expressions of CARMA3 mRNA in cholangiocarcinoma HUCCT1 and RBE cells as well as in normal biliary epithelia cell line HIBEC were determined by qRT-PCR. After silencing the expression of CARMA3 with si RNA technique, the changes in proliferation, apoptosis, cell cycle and abilities of migration and invasion in HUCCT1 and RBE cells were examined by CCK-8 assay, flow cytometry and Transwell assay, respectively. Results： The expression levels of CARMA3 mRNA were significantly increased in both cholangiocarcinoma cell lines compared with normal biliary epithelia cell line HIBEC（HUCCT1 vs. HIBEC： t=5.321, P=0.011; RBE vs. HIBEC： t=5.932, P=0.008）. In both cholangiocarcinoma cell lines after silencing the expression of CARMA3, the abilities of proliferation, metastasis and invasion were significantly inhibited, and the G_1-phase arrest and apoptosis rates were significantly increased（all P〈0.05）.Conclusion： CARMA3 is up-regulated in cholangiocarcinoma cells, and its actions may be associated with promoting cell proliferation, migration and invasion, and inhibiting cell apoptosis.