响应面法优化金银花绿原酸提取工艺研究

Optimization of extraction process of honeysuckle chlorogenic acid by response surface methodology

以金银花为实验材料,为获取金银花中绿原酸提取的最优工艺条件。在单因素实验的基础上,选择提取温度、液料比(V_水/M(金银花))、提取时间为影响因素,以绿原酸提取率为响应值,采用Box-Behnken实验设计对提取工艺建立数学模型进行响应面分析。结果表明,绿原酸最佳提取工艺为:提取时间34.30 min,提取温度81℃,提取液料比24.2,绿原酸提取率为4.38%,较未优化时提高12.31%。

In this paper,honeysuckle was used as the experimental material to obtain the optimum technique of extraction of chlorogenic acid from honeysuckle. On the basis of single-factor test,the extraction temperature,liquid-to-material ratio（Vwater/Mhoneysuckle）and extraction time were selected as the influencing factors;the chlorogenic acid extraction rate was selected as the response value,and the Box-Behnken experimental design was used to establish the mathematical model of extraction process for the response surface analysis. The results showed that the optimum extraction process of chlorogenic acid was ：extraction time 34.30 min,extraction temperature 81℃ and extraction liquid-to-material ratio 24.2,the chlorogenic acid extraction rate was 4.38%,which increased 12.31% compared to unoptimized one.