毛竹NF-Y家族基因的鉴定与表达分析

Genome-wide Identification and Expression Analysis of the NF-Y Family Genes in Phyllostachys edulis

为阐明毛竹NF-Y家族基因的结构、进化关系和表达情况,本研究利用水稻和拟南芥NF-Y家族的蛋白序列,通过Blast比对分析,鉴定出33个毛竹NF-Y家族基因。基于植物NF-Y亚家族成员的氨基酸序列特征,毛竹33个NF-Y家族成员分为3个亚家族,分别命名为Ph NF-YA、Ph NF-YB和Ph NFYC。构建毛竹、二穗短柄草、粟、水稻和拟南芥NF-Y家族系统发育树,结果表明,毛竹各NF-Y亚家族成员的进化趋势不同,与Ph NF-YA亚家族相比,Ph NF-YB和Ph NF-YC在进化上较为保守,毛竹在NF-YA亚家族上可能出现了扩增,而在NF-YB和NF-YC两个亚家族上可能出现了成员丢失现象。通过Cufflinks等软件对毛竹7个组织材料的转录本深度测序数据分析,发现有些NF-Y家族基因在各器官中普遍表达,如Ph NF-YA7、Ph NF-YA3和Ph NF-YB8在根茎叶中均有表达;有些NF-Y家族基因则在特定器官表达,如Ph NF-YA1、Ph NF-Y2、Ph NF-YA4和Ph NF-YA10在竹笋幼根表达。实时荧光定量RT-PCR分析毛竹NF-Y家族基因在毛竹笋各个组织的表达情况,结果表明,毛竹NF-Y家族基因在不同的节间组织中差异表达。本研究结果为进一步深入探究毛竹NF-Y家族基因功能奠定了理论基础。

In order to analysis the structure,evolution pattern,and expression pattern of Phyllostachys edulis NF-Y family genes,in this study,33 NF-Y family genes were identified in Phyllostachys edulis by blast（basic local alignment search tool） with Oryza sativa and Arabidopsis thaliana NF-Y protein sequences as query. All 33 members are divided into three subfamilies,Ph NF-YA,Ph NF-YB and Ph NF-YC by reference to the characteristics of three NF-Y subfamily sequences. The phylogenetic trees constructed by NF-Y family members of Ph. edulis,Brachypodium distachyon,Setaria italica,O. sativa and A. thaliana,show that NF-Y family members of Ph. edulis have different evolution trends. Ph NF-YB and Ph NF-YC subfamily are more conservative in evolution,compared with Ph NF-YA subfamily. And Ph NF-YA subfamily may be amplified,while in Ph NF-YB and Ph NF-YC subfamily members may be lost. Transcription sequencing data of seven tissues of Ph. edulis was downloaded and analyzed using Cufflinks software. The results show that some NF-Y family genes are expressed in all seven tissues,e. g. Ph NF-YA7,Ph NF-YA3 and Ph NF-YB8 were expressed in the roots,stems and leaves; and others were expressed in specific organs e. g. Ph NF-YA1,Ph NF-Y2,Ph NF-YA4 and Ph NF-YA10 were expressed only in the roots. In order to analyze the contribution of NF-Y family genes to bamboo shoot development,the mRNA from different Ph. edulis shoot internode tissues were extracted and RT-q PCR results showed that the Ph. edulis NF-Y family genes were diversely expressed in different internode tissues. All in all,we systematically investigated the structure of NF-Y family genes of Ph. edulis,phylogenetic relationship and the expression pattern of these genes,which lays a base for the comprehensive study of Ph. edulis NF-Y family gene function in the future.