氮离子注入诱变选育大豆疫霉高效拮抗菌

Mutagenesis Breeding of High-efficiency Antagonistic Bacteria of Phytophthora sojae by Nitrogen Ion-implantation

为获得生防效果更佳的芽孢杆菌和荧光假单胞菌菌株,本试验采用低能N+离子注入技术对DDGJ05、DDGJ01和DDEN01菌株进行诱变,并结合平板对峙培养法对N+离子注入诱变后的菌株进行筛选。结果表明,只有DDGJ05有正突变菌株的出现,正突变菌株的编号分别为DDGJ05-20-8、DDGJ05-40-8、DDGJ05-40-10。平板对峙培养试验结果表明,DDGJ05-20-8、DDGJ05-40-8、DDGJ05-40-10对大豆疫霉菌丝生长的抑制率分别为91.12%、93.52%和94.00%,均高于对照亲本菌株DDGJ05。继代培养试验结果表明,正突变菌株DDGJ05-40-8和DDGJ05-40-10对大豆疫霉的拮抗作用能够稳定遗传。采用盆栽法研究了DDGJ05和3个正突变菌株对大豆疫病的控制效果,结果表明,大豆种子经DDGJ05菌株及其3个正突变菌株处理后,对大豆疫病均有一定的防治效果,其发病严重程度均低于空白对照组,其中,经DDGJ05-40-10处理的防治效果最好,防治效果为63.43%。本研究结果为芽孢杆菌离子束注入诱变育种方法的研究提供了理论支撑。

To improve the antagonistic ability of Bacillus subtillis and Pseudomonas fluorescens, three strains DDGJ05, DDGJ01 and DDEN01were induced by low energy N＋ ion beam implantation technology, and the mutation strains were screened by dual culture test. The mutagenic results revealed that DDGJ05 had positive mutation strains, and there were DGJ05-20-8 ,DDGJ05-40-8 and DDGJ05 - 40 - 10. Dual culture test showed that the inhibitory rates of DGJ05 - 20 -8 ,DDGJ05 -40 -8 and DDGJ05 -40 - 10 strains against the mycelial growth of P. sojae were 91. 12% , 93.52% and 94.00% respectively, higher than the parental strain DDGJ05. The heredity characters of DDGJ05 - 40 - 8 and DDGJ05-40 -10 were stable. The effects of the three strains controlling Phytophthora root rot were tested by pot experiment. The results indicated that the soybean seeds treated with these trains could reduce the disease. All above results indicated that the strain DGJ05 - 20 - 8 ,DDGJ05 - 40 - 8 and DDGJ05 - 40 - 10 could effectively inhibit the mycelial growth of P. sojae and reduce the disease occurrence. The results provided the foundation for the study of ion beam implantation mutation on Bacillus subtilis.