绵羊STMN1基因的克隆、序列分析及其组织表达

Cloning,Sequence Analysis and Tissue Expression of STMN1 Gene in Sheep(Ovis aires)

为阐明STMN1功能及其对绵羊肌肉生长发育的影响,本研究克隆了绵羊STMN1基因序列,对该基因编码蛋白质的理化性质进行预测,分析其在绵羊不同组织及不同发育时期背最长肌和股二头肌的表达特性。结果表明,克隆得到绵羊STMN1基因CDS区长450 bp,编码149个氨基酸,蛋白质分子量为17 302.51 D,等电点为5.75;绵羊STMN1氨基酸序列与山羊、牛、猪、小鼠和人的相似性达到90%以上;系统进化树分析表明,绵羊STMN1基因与山羊的亲缘关系最近。STMN1在绵羊睾丸中表达量最高,心和脾表达量次之,肝、肺、肾、背最长肌和股二头肌表达量较低。STMN1在不同发育阶段的绵羊背最长肌和股二头肌中均有表达,在绵羊背最长肌中,24月龄绵羊中STMN1高表达,0、2、5、12月龄中度表达;在绵羊股二头肌中,5月龄绵羊中STMN1高表达,2月龄中度表达,0、12、24月龄低表达。本研究结果为进一步阐明STMN1基因在绵羊中的生物学功能奠定了理论基础。

In order to clarify STMN1 function and its influence on the growth and development of sheep muscle,this study cloned the gene sequence of sheep STMN1,predicted the physicochemical properties of the gene encoding protein,analyzed its expression in the different tissues and different development stages of longissimus dorsi and biceps femoris muscle of sheep. The results showed the CDS length of sheep STMN1 gene was 450 bp,encoded 149 amino acid,the molecular weight of protein was 17 302. 51 D,and the isoelectric point was 5. 75. Sheep STMN1 amino acid sequence was move than 90% similar to goat,cattle,pig,mice and human. Phylogenetic tree showed that sheep STMN1 gene had the closest relationship with goat. The highest expression of STMN1 was in testis,followed by heart and spleen,and its expression in liver,lung,kidney,longissimus dorsi and biceps femoris muscle was the lowest. STMN1 expressed in both longissimus dorsi and biceps femoris muscle of sheep in different development stages. In the longissimus dorsi muscle of sheep,STMN1 highly expressed at the age of 24 month,and it also moderately expressed at 0,2,5 and 12 months of age. In the biceps femoris muscle of sheep,STMN1 highly expressed at the age of 5 months,and it was moderately expressed at the age of 2 months,and it had a low expression at the age of 0,12 and 24 months. The results of this study provide a theoretical basis for further clarifying the biological function of STMN1 gene in sheep.