阿里红多糖组分对小鼠脾淋巴细胞免疫功能的影响

Effects of polysaccharide from Fomes officinals Ames on immune function of murine splenic lymphocytes

目的研究阿里红多糖组分FOPS-a、FOPS-b对小鼠脾脏淋巴细胞免疫调节功能的影响。方法实验方法选用CCK-8(Cell Counting Kit-8)试剂检测不同质量浓度阿里红多糖组分对小鼠脾脏淋巴细胞增殖率的影响;Griess法测定阿里红多糖组分对NO的释放量的影响;ELISA试剂盒测定阿里红多糖组分对IL-2、IL-4、INF-γ和IGg释放能力的影响。结果一定质量浓度的阿里红多糖组分能促进淋巴细胞的增殖活性,刺激淋巴细胞释放NO,并促进淋巴细胞分泌IL-2、IL-4、INF-γ和IGg。结论阿里红多糖组分在体外具有一定的免疫活性,能增强小鼠脾脏淋巴细胞免疫功能。

Objective To study the effects of polysaccharide components FOPS-a and FOPS-b from Fomes officinals Ames on immunomodulatory function of spleen lymphocytes in mice. Methods The effects of different mass concentrations of Fomes officinals Ames polysaccharide components on proliferation rate of spleen lymphocytes in mice were detected by Cell Counting kit-8 reagent, the effect on the release of NO was determined by Griess method, and the effects on the release capacity of IL-2, IL-4, INF-γ and IGg were determined by the ELISA kit. Results The polysaccharide components of Fomes officinals Ames with a certain mass concentration could promote the proliferation activity of lymphocytes, stimulate lymphocytes to release NO and promote lymphocytes to secrete IL-2, IL-4, INF-γ and IGg. Conclusion Fomes officinals Ames polysaccharide components have certain immune activity in vitro and can enhance the immune function of spleen lymphocytes in mice.