外周血血管生成素-2mRNA定量表达与食管癌侵袭转移及预后的关系

Correlation between quantitative expression of Angiopoietin-2mRNA in peripheral blood and invasion and metastasis and prognosis of patients with esophagus cancer

[目的]检测食管癌患者术前与术后外周血血管生成素-2(Angiopoietin-2,Ang-2)mRNA表达量的变化,探讨其与食管癌侵袭转移及预后的关系。[方法]应用实时荧光定量RT-PCR检测68例食管癌患者根治术前及术后外周血Ang-2mRNA表达量变化,术后3年预后随访,分析外周血Ang-2mRNA表达量变化与食管癌侵袭转移及预后的关系。选择25例健康志愿者为正常对照组。[结果]食管癌组术前外周血Ang-2mRNA表达量显著高于正常对照组(P<0.01)。食管癌组术后1个月时外周血Ang-2mRNA表达量较术前显著下降(P<0.01),中晚期食管癌患者术前与术后1个月时外周血Ang-2mRNA表达量均显著高于早期食管癌患者(P<0.01);术前外周血Ang-2mRNA表达量与肿瘤大小、有无淋巴结转移及浸润深度均呈正相关(P<0.05或0.01),而与组织学类型、肿瘤部位无相关性(P>0.05),与肿瘤分化程度、术后生存期、术后复发转移及术后生存率呈负相关(P<0.05或P<0.05或0.01)。多因素分析显示术前外周血Ang-2mRNA表达量是影响食管癌患者预后的高危因素。[结论]术前外周血Ang-2mRNA高表达促进食管癌侵袭转移,与不良预后相关,可能成为其预后评价指标。

[Objective]To investigate the relationship between quantitative expression of Angiopoietin-2 （Ang-2） mRNA in peripheral blood and invasion,metastasis,prognosis of patients with esophagus cancer. [Methods]The preoperative expression levels of Ang-2mRNA in peripheral blood in 68patients with esophagus cancer and 25normal controls were measured by real-time quantitative RT-PCR respectively. The 3years postoperative prognosis was followed-up. The relation between the preoperative expression levels of Ang-2mRNA in peripheral blood and invasion,metastasis,prognosis in the patients with esophagus cancer was investigated. [Results]The expression levels of Ang-2mRNA in peripheral blood in the patients with esophagus cancer were significantly higher than that in the normal controls（P〈0.01）. The preoperative expression levels of Ang-2mRNA in peripheral blood in the patients with esophagus cancer were positively related with the tumor size,lymphatic metastasis, depth of invasion（P〈0.05 or〈0.01）, but not with the histological type, tumor site, and were negatively related with the differentiated degree of tumor, postoperative survival time, survival rate and the postoperative recurrence and metastasis （P〈0.05 or 0.01） .Multivariate analysis revealed that preoperative expression levels of Ang-2mRNA in peripheral blood were independent prognostic factor for the patients with esophagus cancer. [Conclusion]Preoperative high expression levels of Ang-2mRNA in peripheral blood promote invasion and metastasis of esophagus cancer, are closely relative with the poor prognosis,may be a prognostic factor for esophagus cancer.