纤维素酶解反应动力学的研究

Research of Reaction Kinetics of Cellulose Enzymolysis

为研究纤维素酶解反应动力学,建立了纤维素酶解反应动力学模型,以玉米秸秆采用中石化螺杆连续汽爆装置预处理得到的物料为实验原料,在20%底物浓度、酶加量是每g纤维素中加入0.05 g诺维信CTec3酶、酶解停留时间72 h的条件下进行纤维素酶解实验,再根据实验结果对纤维素酶解反应动力学模型中的参数进行求解,最终结论为酶解得率x和酶解停留时间t之间的纤维素酶解反应动力学方程为(1-x)-2-1=0.329 3,并对其准确性和可预测性进行了验证。该研究工作为纤维素酶解反应器的开发和放大奠定理论基础。

To study cellulase hydrolysis kinetics, the model was established in this article. With corn straw which was pretreated by using SINOPEC screw continuous steam explosion devise as the experimental material, cellulase hydrolysis experiment was carried out under the condition of 20% concentration of substrate, 1 g of cellulas containing 0.05 g of Novozymes CTec3 enzyme, and 72 h of holding time. Based on the results from the experiment, the parameters in the model of cellulase hydrolysis kinetics were solved. The cellulase hydrolysis kinetics equation for linking yield rate of enzymatic hydrolysis x with holding time t is：（1-x）-2-1=0.329 3, and the accuracy and predictability of this equation had been validated. The work presented herein can be used as the basis for the development and amplification of cellulase hydrolysis reactor.