仿生金线莲有效成分含量及影响因素研究

Contents of Medicinal Components in Anoectochilus Roxburghii and Their Related Factors Under Eco-Cultivation Pattern

为探讨仿生栽培过程金线莲品质变化,对不同生长时期金线莲有效成分、环境因子及它们之间的关系进行了研究。结果表明:栽培期间,仿生金线莲多糖含量略有增加,与野生金线莲含量近似;游离氨基酸含量先下降再上升,最终接近野生金线莲含量水平;仿生金线莲苯丙氨酸解氨酶(P A L)活性变化幅度较大,大部分时期高于野生金线莲;黄酮含量变化呈先减后增,再缓慢减少的趋势,栽培5-6个月后达到最高水平。相关分析显示,仿生金线莲生理生化指标与环境因子相关性不显著,仅P A L酶活性与空气温、湿度显著相关,对环境变化较为敏感。研究表明,仿生种植金线莲主要药用成分含量与野生金线莲相仿,每年9-1 0月是仿生金线莲适宜的采收时期。

To explore quality changes of Anoectochilus roxburghii under ecological cultivation pattern active ingredients and their rela- tions with environmental factors were investigated. The result showed polysaccharide contents increased slightly in ecologically cultivated A. roxburghii, which approximate to the level of wild A. roxburghii. Free amino acid contents declined firstly, but increased subsequently and were close to the content of wild A. roxburghii at the end. Although phenylalanine ammonia-lyase （PAL） activities changed intensively PAL activities were higher generally in ecologically cultivated A. roxburghii than in wild species. Flavonoid contents took on a tendency of dropping first and then rising. After that, flavonoid contents reduced mildly. Flavonoid contents approached its maximum after 5-6 months eco-cultivation. Con＇elation analysis indicated most biochemical indexes of ecologically cultivated anoectochilus roxburghii had no obvious correlation with environmental factors. Only PAL activities related to air temperature and humidiU significantly, which displayed its sensitivity on environmental changes. It could conclude the contents of main medicinal components were similar in eco-cultivation and wild Anoectochilus roxburghii. The best harvest time of ecologically cultivated A. roxburghii was from September to October.