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基于双光子共聚焦成像的大鼠小梁网三维重建及有限元分析 被引量:1

Three-Dimensional Reconstruction and Finite Element Analysis of Rat Trabecular Meshwork Basing on Two-Photon Microscope Images
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摘要 青光眼是一组威胁视神经视觉功能、与眼压升高密切相关的眼病。目前认为临床上大部分眼压的升高与房水流出阻力增加有关,特别是小梁网流出阻力的增加。因此,研究高眼压下影响房水外流阻力的重要区域-小梁网的形态学信息尤为重要。通过前房灌注的方法制造大鼠急性高眼压动物模型,将6只SD大鼠分成两组(A组和B组),B组大鼠处死后于左眼球加压60 mm Hg处理,其余眼球均为未加压对照组,利用双光子共聚焦成像系统采集正常眼压和高眼压下小梁网组织的断层序列图,通过图像处理方法,定量分析眼压对小梁网组织孔隙率变化的影响。通过三维重建获取正常眼压下的小梁网结构模型,并利用有限元方法,探讨眼压对于小梁网组织形态结构的影响。结合实验数据与模拟计算的结果,综合分析眼压的变化对于小梁网外流阻力的影响。高眼压组数据显示,部分小梁与周围组织融合,胶原纤维出现塌缩,越是靠近前房的小梁组织损伤越严重。有限元分析结果表明,孔隙越多的区域变形越大,而且压力越大,小梁变形程度越明显。眼内压升高可能会引起房水外流通道结构发生异常,主要表现为小梁网胶原纤维发生塌缩。高眼压与正常眼压情况相比,小梁网区域外流阻力增大的可能性较大。 Glaucoma is a disease of irreversible blindness with the most risk factors of ocular hypertension that causes damage to the visual function and optic nerve. So far,clinical evidence indicates that increased intraocular pressure( IOP) is a function of elevated resistance to drainage of aqueous humor,especially the increase of outflow resistance within the trabecular meshwork( TM). Therefore,it is very important to figure out what effects of high IOP on the structures of TM and analyze the morphologic changes of TM contributing to the increase of outflow resistance in turn. In this study,animal model of acute IOP of rat was established by methods of anterior chamber perfusion. Six SD rats were divided into A,B two groups. After being killed,the left eyes of the SD rats in group B were perfused at pressure of 60 mm Hg for imaging under the condition of ocular hypertension,and the other eyes was used as the control group. The tomographic sequence images of TM were obtained in the condition of normal or high IOP by using two-photon microscopy. The effects of IOP on the TM porosity based on image processing were quantitatively investigated. The three-dimensional model of TM was obtained in the normal IOP eye,and the morphological changes of TM were analyzed under different intraocular pressures by using the finite element method. The effect of high IOP on the outflow resistance of TM was analyzed by using the experiment integrated with the simulation method. In the high IOP group,the TMbeams became collapsed and merged with surrounding tissues,and the damage of TM tissue near the anterior chamber is more serious. Through the analysis of finite element method,it was found out that the biggest deformation was located in the region that the porosity of TM was larger. Meanwhile,it was indicated that the greater the pressure was,the greater the damages of TM beams was. Eyes with the high IOP may cause the structural changes of TM. Fibers of TM were collapsed. The possibility of increasing outflow resistance could occur in high IOP eyes but was much smaller in normal eyes.
作者 张静 钱秀清 张海霞 任琳 刘志成 Zhang Jing;Qian Xiuqing;Zhang Haixia;Ren Lin;Liu Zhicheng(Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application,School of Biomedical Engineering,Capital Medical University,Beijing 100069,China)
出处 《中国生物医学工程学报》 CAS CSCD 北大核心 2018年第4期461-467,共7页 Chinese Journal of Biomedical Engineering
基金 国家自然科学基金(31570952,81471702) 北京自然科学基金(3122010)
关键词 小梁网 有限元分析 高眼压 青光眼 trabecular meshwork finite element analysis high intraocular pressure glaucoma
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