子宫肌瘤发病相关因素分析及对Ki-67、P16和P53表达研究

Analysis on the related factors of uterine fibroids and the expressions of Ki67,P16,and P53

目的探讨子宫肌瘤发病相关因素及Ki-67、P16和P53表达,为临床早发现和早治疗子宫肌瘤提供参考。方法选择2015年6月-2017年6月芜湖市第二人民医院收治的218例子宫肌瘤患者为研究对象(观察组),同期100例正常子宫组织标本为对照组。调查两组年龄、BMI、产次、流产史、痛经、遗传病史、初潮年龄、月经紊乱、避孕药、生殖道炎等,采用单因素和多因素Logistic回归分析影响子宫肌瘤独立危险因素。采用免疫组化法测定Ki-67、P16和P53表达阳性率。结果单因素研究显示,两组年龄、BMI、产次、痛经、遗传病史以及初潮年龄比较,差异无统计学意义(P>0.05),流产史、月经紊乱、避孕药以及生殖道炎比较,差异有统计学意义(P<0.05)。将上述单因素分析具有统计学差异的纳入Logistic回归分析,流产史、月经紊乱、避孕药和生殖道炎为影响子宫肌瘤独立危险因素。观察组Ki-67、P16和P53表达阳性率高于对照组,两组比较,差异有统计学意义(P<0.05)。结论子宫肌瘤发病受多种因素影响,应采取针对性预防性措施避免子宫肌瘤发生,且子宫肌瘤患者Ki-67、P16和P53呈高表达。

Objective To explore the related factors of uterine fibroids and the expressions of Ki67,P16,and P53,provide a reference for clinical early detection and early treatment of uterine fibroids. Methods From June 2015 to June 2017,218 patients with uterine fibroids treated in the Second People＇s Hospital of Wuhu City were selected as observation group,and 100 women with normal cervical tissue were selected as control group during the same period. Age,body mass index（ BMI）,parity,abortion history,dysmenorrhea,history of genetic diseases,menarche age,menstrual disorder,use of contraceptive drugs,and genital tract inflammation in the two groups were surveyed. Univariate and multivariate logistic regression models were used to analyze the independent risk factors of uterine fibroids. Immunohistochemistry was used to detect the positive expression rates of Ki-67,P16,and P53. Results Univariate analysis showed that there was no statistically significant difference in age,BMI,parity,dysmenorrhea,history of genetic diseases,and menarche age between the two groups（ P〉0. 05）. There were statistically significant differences in abortion history,menstrual disorder,use of contraceptive drugs,and genital tract inflammation between the two groups（ P〈0. 05）. The factors with statistic difference in univariate analysis were involved in multivariate logistic regression analysis,abortion history,menstrual disorder,use of contraceptive drugs,and genital tract inflammation were independent risk factors of uterine fibroids. The positive expression rates of Ki-67,P16,and P53 in observation group were statistically significantly higher than those in control group（ P〈0. 05）. Conclusion The occurrence of uterine fibroids is affected by many factors. The preventive measures should be taken to avoid the occurrence of uterine fibroids,the expression rates of Ki-67,P16,and P53 in patients with uterine fibroids are high.