FOXD3的空间结构和理化性质分析

Analysis of the spatial structure and physicochemical property of FOXD3

FOXD3(Forkhead box D3)作为一个有价值的肿瘤预后标志物,其抑癌机制和预后价值仍未被完全阐明。为了全面认识FOXD3,本研究利用生物信息学手段,对FOXD3蛋白的氨基酸序列同源性、理化性质、组织表达、亚细胞定位、空间结构及蛋白质相互作用网络进行了预测和分析。结果表明:人FOXD3蛋白是酸性不稳定的亲水蛋白,在进化过程中高度保守,无信号肽和跨膜区域,属于FH超家族。该蛋白定位于细胞核的可能性最大,主要二级结构为无规卷曲。经GO分析和KEGG通路分析可知,与FOXD3相互作用的蛋白主要是转录因子,参与调控细胞干性的蛋白质和调控肿瘤细胞特性的蛋白质。本文结果为进一步研究FOXD3的功能及抑癌机制提供一定的参考。

Forkhead box D3 （FOXD3） is a valuable prognostic marker for different kinds of tumors.However,the tumor suppression mechanism and prognostic value of FOXD3 have not been fully elucidated. To gain insightful information of FOXD3 protein, we selected multiple bioinformatics methods to analyze the hereditary conservation, chemical properties, tissue expression, subcellular localization, space structure, and protein interaction networks of FOXD3 protein. The results showed that the FOXD3 protein is acidic, unstable, and hydrophilic without signal peptide and trans-membrane region. It is highly conserved and belongs to the FH super family. FOXD3 is mainly located in the nucleus and the main secondary structure elements are random coil. GO and KEGG enrichment analyses show that interactive proteins with FOXD3 are mainly transcription factors, namely, proteins involved in regulating the stemness feature of cells and proteins regulating the property of cancer cells. Our results provide some reference for further study of the function and tumor suppression mechanism of FOXD3 protein.