摘要
目的探讨强直性脊柱炎(AS)患者人类白细胞抗原B27(HLA-B27)各亚型分布及其与疾病的相关性。方法收集103例AS及105例健康对照患者血清,流式细胞术初筛HLA-B27抗原,聚合酶链式-直接测序分型法(PCRSBT)测定基因型别。结果 AS组检出4种HLA-B27亚型,分别为B2704、B2705、B2707和B2715,B2704占HLA-B27亚型构成比最高(93.93%)。结论本地区AS患者HLA-B27基因型别以B2704亚型为主。
Objective To study the distribution of gene subtype of human leukocyte antigen B27(HLA-B27)with ankylosing spondylitis(AS)and analyze their association.Methods The antigen and gene of HLA-B27 were detected by flow cytometry(FCM)and polymerase chain reaction-sequence based typing(PCR-SBT)in 103 cases with AS and 105 health control.Results Four subtypes of HLA-B27(B2704,B2705,B2707 and B2715)were found in the AS group.Among these four alleles,B2704 was the most widespread subtype,accounting for 93.93%(93/100).Conclusion The B2704 is the most wide spreadsubtype with AS in Fujian area.
作者
刘巧灵
范春梅
LIU Qiaoling;FAN Chunmei(Department of center laboratory,the second affiliated hospital of Fujian Medical University,Quanzhou,Fujian 362000,China)
出处
《福建医药杂志》
CAS
2018年第4期26-29,共4页
Fujian Medical Journal