多巴胺受体在大鼠丙泊酚全身麻醉苏醒过程中的作用

Role of dopamine receptors in the emergence from propofol general anesthesia in rats

目的评价多巴胺受体在大鼠丙泊酚全身麻醉苏醒过程中的作用。方法健康雄性SD大鼠50只随机分为对照组(C组)、多巴胺1型(D1)受体激动剂Chloro-APB组(Chloro-APB组)、D1受体拮抗剂SCH-23390组(SCH-23390组)、多巴胺2型(D2)受体激动剂Quinpirole组(Quinpirole组)及D2受体拮抗剂Eticlopride组(Eticlopride组)五组。以翻正反射消失(LORR)时间评价麻醉起效时间、翻正反射恢复(RORR)时间评价麻醉苏醒时间,以共济失调期评价运动功能恢复。各组大鼠均通过尾静脉注射丙泊酚(11 mg/kg),并记录大鼠LORR时间。五组大鼠在丙泊酚全身麻醉后静脉注射生理盐水稀释的如下试剂共0.5 m L:C组注射生理盐水0.5 m L;Chloro-APB组注射D1受体激动剂Chloro-APB(3mg/kg);SCH-23390组注射D1受体拮抗剂SCH-23390(0.2 mg/kg);Quinpirole组注射D2受体激动剂Quinpirole(5 mg/kg);Eticlopride组注射D2受体拮抗剂Eticlopride(0.2 mg/kg),并记录大鼠RORR时间。待大鼠RORR后,记录共济失调期。结果五组大鼠丙泊酚麻醉后LORR时间差异无统计学意义(P>0.05)。与C组比较,Chloro-APB组丙泊酚麻醉后RORR明显缩短(P<0.05);SCH-23390组丙泊酚麻醉后RORR明显延长(P<0.05);Quinpirole组丙泊酚麻醉后RORR差异无统计学意义(P>0.05);Eticlopride组丙泊酚麻醉后RORR差异无统计学意义(P>0.05)。五组大鼠RORR后,共济失调期差异无统计学意义(P>0.05)。结论多巴胺1型受体激活后可明显缩短大鼠从丙泊酚全身麻醉苏醒的时间。

Objective To determine the role of dopamine receptors in the process of emergence from propofol general anesthesia in rats. Methods Fifty male Sprague-Dawley rats were divided into 5 groups（ n = 10 each） using a random number method which were control group（ group C）,dopamine type 1 receptor agonist Chloro-APB group（ group ChloroAPB）,dopamine type 1（ D1） receptor antagonist SCH-23390 group（ group SCH-23390）,dopamine type 2（ D2） receptor agonist Quinpirole group（ group Quinpirole）,D2 receptor antagonist Eticlopride group（ group Eticlopride）. The time of loss of righting reflex（ LORR） were used to evaluate the onset time,the time of resumption of righting reflex（ RORR）were used to estimate the recovery time,the duration of post-LORR ataxia were used to evaluate the motor function. Corresponding reagents were dissolved in normal saline to a final volume of 0. 5 m L,and administered intravenously via the lateral tail vein. Firstly,the time of LORR was recorded after intravenous injection of propofol（ 11 mg/kg） in five groups.Next,the time of RORR was recorded after intravenous injection of saline（ 0. 5 ml） in group C（ n = 10）. In Chloro-APB（ D1 receptor agonist） group（ n = 10）,recording the time of RORR after rats were injected with Chloro-APB（ 3 mg/kg）.In SCH-23390（ D1 receptor antagonist） group（ n = 10）,recording the time of RORR after rats were injected with SCH-23390（ 0. 2 mg/kg）. In Quinpirole（ D2 receptor agonist） group（ n = 10）,recording the time of RORR after rats were injected with quinpirole（ 5 mg/kg）. In eticlopride（ D2 receptor antagonist） group（ n = 10）,recording the time of RORR after rats were injected with eticlopride（ 0. 2 mg/kg）. After resumption of righting reflex,recording the duration of post-LORR ataxia. Results No significant changes were found in the time of LORR after intravenous of propofol（ P〈0. 05） among the five groups. In Chloro-APB group,compared with C group,intravenous injection of Chloro-APB decreased the time of RORR（ P〈0. 05）. In contrast,SCH-23390 group compared with C group,intravenous injection of Chloro-APB increased the time of RORR（ P〈0. 05）. Moreover,in Quinpirole group and Eticlopride group,compared with C group respectively,no significant changes were found in the time of RORR（ P〉0. 05）. No significant changes were found in the duration of post-LORR ataxia after RORR（ P〉0. 05） among the five groups. Conclusion Activation of D1 dopamine receptors facilitates emergence from propofol general anesthesia in rats.