葛根素对重度烧伤后肺组织损伤保护作用的研究

Protective effect of puerarin on lung tissue injury after severe burns

目的研究葛根素对大鼠重度烧伤后肺组织损伤的保护作用及其可能的作用机制。方法取180只实验用大鼠,按照随机数字表法随机分为假烧伤组,模型组,葛根素低(50 mg/kg)、中(100 mg/kg)、高(200mg/kg)剂量组和地塞米松5 mg/kg组(地塞米松组),各30只。采用92℃水浴18 s的方法制备30%TBSAⅢ度烧伤大鼠模型;测定肺组织湿/干重比(W/D),HE染色进行肺组织病理学检查并进行肺泡炎评分(Szapiel评分),电子显微镜观察肺组织超微结构变化,TUNEL染色观察细胞凋亡状况并计算凋亡指数(Apoptosis index,AI),测定肺组织中抗氧化酶、髓过氧化物酶(MPO)活性和MDA含量,测定血清中炎症细胞因子含量水平。结果与模型组比较,葛根素中、高剂量组和地塞米松组W/D显著降低(P <0. 05或P <0. 01);肺组织病理学改变和超微结构均明显改善,Szapiel评分显著降低(P <0. 05);肺组织细胞凋亡状况明显改善,AI显著降低(P <0. 01);肺组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、MPO活性明显升高,而MDA含量显著降低(P <0. 05或P <0. 01);血清肿瘤坏死因子(TNF-α)、白细胞介素(IL)-6、IL-8含量显著降低,而IL-10含量显著升高(P <0. 05或P <0. 01)。结论葛根素对大鼠重度烧伤后肺组织损伤具有一定的保护作用,其机制可能与葛根素能够降低氧化应激损伤、抑制炎症反应有关。

b Objective To investigate the effects of puerarin on lung tissue injury in severely burned rats and its mechanism. Methods Totally 180 rats were randomly divided into six groups：sham burn group,model group,puerarin low-（50 mg/kg）, medium-（100 mg/kg）, high-（200 mg/kg） dose group and dexamethasone 5 mg/kg group （dexam- ethasone group） ,n =30. The model rats with 30% TBSA （third degree） were made by bathing in 92 t water for 18 s. The wet/dry （ W/D） ratio of lung tissue were examined; the histopathological changes of lung tissue were ob-served and the Szapiel score were determined ; the ultrastructural changes of lung tissue were observed by transmission electron microscope; the apoptosis of the lung tissue cells was observed by TUNEL staining and the AI was determined; the activity of antioxidant enzymes, the activity of myeloperoxidase （ MPO） and the contents of MDA in the lung tissue were measured,the level of inflammatory cytokines in serum was determined. Results Compared with model group, the W/D of the lung tissue in puerarin medium- and high-dose group and dexamethasone group was significantly de-creased （P 〈0.05 or P 〈0. 01） , the histopathological changes and ultrastructural changes of lung tissue were signifi-cantly improved,and the Szapiel score was significantly decreased （ P 〈 0 .0 5 ） ; the apoptosis of the lung tissue cells was significantly improved and the AI was significantly decreased （ P 〈0. 01）; the activity of SOD, CAT and MPO in lung tissue was significantly increased, while the content of MDA was significantly decreased （P 〈0.05 or P 〈0. 01 ）; the level of TNF-α,IL-6 and IL-8 in serum was significantly decreased,while the IL-10 was significantly increased （P 〈0.05 or P 〈0. 01）. Conclusion Puerarin has protective effects on lung tissue injury in severely burned rats , which is perhaps related to its effects of depressing oxidative stress damage and inflammation.