前列地尔注射液对缺血性结肠炎患者血清肠脂肪酸结合蛋白及二胺氧化酶水平的影响

Effect of alprostadil injection on serum IFABP and DAO in patients with ischemic colitis

目的观察前列地尔注射液对缺血性结肠炎(Ischemic colitis,IC)患者血清肠脂肪酸结合蛋白(Intestinal fatty acid binding protein,IFABP)和二胺氧化酶(Diamine oxidase,DAO)的影响。方法 76例IC患者随机分为治疗组(39例)和对照组(37例),对照组给予IC常规治疗,治疗组在常规治疗基础上加用前列地尔注射液10μg静脉滴注,1次/d,疗程为2周。观察两组的疗效。采用酶联免疫吸附试验法(ELISA)测定治疗前后血清IFABP、DAO水平,并与30例健康体检者进行对比。分析血清IFABP、DAO水平与疗效的相关性。结果最终有69例患者完成了本研究,脱漏7例(对照组34例,脱漏3例;治疗组35例,脱漏4例)。治疗前,两组患者血清IFABP、DAO水平比较差异无统计学意义(P> 0. 05),但与健康对照组比较,两组患者血清IFABP、DAO水平升高(P <0. 01)。治疗后,两组患者血清IFABP、DAO水平均下降(P <0. 01,P <0. 05),且治疗组IFABP、DAO水平低于对照组(P <0. 05);治疗组的有效率高于对照组(82. 86%vs. 55. 88%,P <0. 05)。相关性分析表明,血清IFABP、DAO水平均与疗效等级呈明显负相关(r=-0. 687、-0. 723,P <0. 01)。结论 IC患者血清IFABP、DAO水平升高,在常规治疗基础上加用前列地尔注射液可提高疗效,其机制可能与其降低血清IFABP及DAO水平、改善肠黏膜功能有关。

Objective To investigate the effect of alprostadil injection on serum IFABP and DAO in patients with ischemic colitis. Methods Totally 76 patients with ischemic colitis were divided into 2 groups randomly. Control group （n =37） received routine treatment for ischemic colitis, while treatment group （ n = 39） were given alprostadil injection （40 μg iv once daily） in addition to routine treatment. The treatment course was 2 weeks. The efficacy was e- valuated after treatment. The levels of serum IFABP and DAO were detected by ELISA before and after treatment. Thir- ty healthy people were included in the healthy control group. The correlation between levels of serum IFABP or DAO and efficacy was analyzed. Results Totally 69 patients completed the study,including 34 cases in control group （3 ca- ses missed） and 35 cases in treatment group （4 cases missed）. There was no significant difference in the levels of ser- um IFABP and DAO before treatment （P 〉 0.05 ） , which were higher than those of healthy control group （P 〈 0.01 ）. After treatment,the levels of serum IFABP and DAO were decreased significantly （P 〈 0.01 ,P 〈 0.05 ）, and those in treatment group were higher than control group （P 〈 0.05 ）. The effective rate in treatment group was higher than that of control group （82.86% vs. 55.88% ,P 〈0.05）. There was a significant negative correlation between the levels of serum IFABP or DAO and degree of efficacy （ r=0. 687, - 0. 723,P 〈 0.01 ）. Conclusion The levels of serum IF- ABP and DAO in IC patients are increased. Alprostadil injection combined with conventional treatment can improve the efficacy ,and the mechanism may be related to decreasing the level of serum DAO and IFABP and improving the intes- tinal mucosal function.