通窍活血汤对大鼠脑缺血再灌注损伤后血清中TNF-α及IL-6水平的影响

Effects of Tongqiao-Huoxue Decoction on Levels of TNF-α and IL-6 in Rats with Cerebral Ischemia Reperfusion Injury

目的:观察通窍活血汤对大鼠脑缺血再灌注损伤后血清中肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白介素-6 (Interleukin-6,IL-6)水平的影响。方法:SD雄性大鼠30只,随机分为假手术组、模型组及通窍活血汤低、中、高剂量组5组。采用线栓法建立大鼠脑缺血再灌注损伤模型,于缺血再灌注24 h后对大鼠进行神经功能损伤评分;酶联免疫吸附剂测定法(ELISA)检测各组血清中TNF-α、IL-6水平。结果:通窍活血汤高、中剂量组可明显减轻大鼠脑缺血再灌注后神经功能损伤和降低血清TNF-α和IL-6的水平。结论:通窍活血汤能够降低大鼠脑缺血再灌注损伤时炎症细胞因子的水平,从而减轻脑缺血再灌注损伤的程度。

Objective： To investigate the effect of Tongqiao-Huoxue Decoctionr on tumor necrosis factor-a （TNF- a ） and interleu -kin-6 （IL-6） after cerebral ischemia reperfusion injury in rat Methods： Thirty healthy male SD rats were randomly divided into 5 groups ： the sham operation group, the model group, Tongqiao-Huoxue Decoction groups of low, middle and high dose groups. Thread block method ＂was used to establish the cerebral ischemia reperfusion injury model in rats. After the reperfusion for 24 h,efforts were made to give neurological score; detect TNF-a and IL-6 content in serum by the method of enzyme-linked immunosorbent assay