活血化瘀方对糖尿病模型大鼠糖脂代谢、血管内皮生长因子和血管紧张素Ⅱ1型受体表达的影响

Effects of Huoxue Huayu Recipe on Glycolipid Metabolism, Vascular Endothelial Growth Factor and Angiotensin Ⅱ1 Type Receptor Expression in Diabetic Rats

目的:探讨活血化瘀方对糖尿病模型大鼠糖脂代谢、血管内皮生长因子(VEGF)和血管紧张素Ⅱ1型受体(AT1R)表达的影响。方法:选取健康雄性SD大鼠50只,适应性喂养7 d后以随机数字表法分成对照组10只、模型组13只、中药组14只、西药组13只。其中模型组与对照组予以纯净水灌胃,中药组予以活血化瘀通络中药配方颗粒灌胃,西药组则予以厄贝沙坦灌胃,1次/d,连续灌胃16周。分别比较各组大鼠的糖脂代谢指标水平及24 h尿蛋白定量、糖化血红蛋白、血清肌酐水平,并检测肾组织VEGF和AT1R表达情况。结果:模型组、中药组、西药组大鼠空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平均高于对照组,中药组、西药组大鼠LDL-C水平低于模型组,中药组大鼠FBG水平低于模型组与西药组(P<0.05)。模型组、中药组、西药组大鼠24 h尿蛋白定量与糖化血红蛋白均高于对照组,中药组、西药组大鼠24 h尿蛋白定量低于模型组(P<0.05)。模型组、中药组、西药组大鼠VEGF、AT1R水平均高于对照组,中药组、西药组大鼠VEGF、AT1R水平低于模型组,中药组大鼠AT1R水平低于西药组(P<0.05)。结论:活血化瘀方可有效改善糖尿病大鼠糖脂代谢状态,通过抑制VEGF与AT1R的表达水平,延缓糖尿病的发生与发展。

Objective： To investigate the effects of Huoxue huayu recipe on glycolipid metabolism,vascular endothelial growth factor（VEGF） and angiotensin Ⅱ1 type receptor（AT1 R） expression in diabetic rats. Methods： A total of 50 healthy male SD rats were selected from the experimental animal center of Hebei province, after adaptive feeding 7 d, the rats were randomly divided into control group（n=10）, model group（n=13）, Chinese medicine group（n=14） and western medicine group（n=13）. The model group and the control group were irrigated the stomach with pure water, the Chinese medicine group was irrigated the stomach with Huoxue huayu recipe,while the western medicine group was irrigated the stomach with erbesartan, once a day, continuous gavage for 16 weeks. The levels of glycolipid metabolism indexes and 24 h urine protein quantification, glycosylated hemoglobin and serum creatinine were compared among the four groups,and the expression of VEGF and AT1 R was examined. Results： The levels of fasting blood glucose（FBG）, total cholesterol（TC）, triglyceride（TG）, high-density lipoprotein cholesterol（HDL-C） and low density lipoprotein cholesterol（LDL-C） in the model group, the Chinese medicine group and the western medicine were all higher than those in the control group, the levels of LDL-C in the Chinese medicine group and the western medicine group were lower than that in the model group, the level of FBG in the Chinese medicine group was lower than those in the model group and the western medicine group（P〈0.05）. 24 h urine protein quantification and glycosylated hemoglobin in the model group, the Chinese medicine group and the western medicine were all higher than those in the control group, 24 h urine protein quantification in the Chinese medicine group and the western medicine group was lower than that in the model group（P〈0.05）. The levels of VEGF and AT1 R in the model group, the Chinese medicine group and the western medicine were all higher than those in the control group, the levels of VEGF and AT1 R in the Chinese medicine group and the western medicine group was lower than that in the model group, and the level of AT1 R in the Chinese medicine group was lower than that of the western medicine group（P〈0.05）. Conclusion： Huoxue huayu recipe can effectively improve the glycolipid metabolic state of diabetic rats, and it can delay the occurrence and development of diabetes through reducing the expression levels of VEGF and AT1R.