一种新型比色荧光增强型CN^-探针的合成与性能研究

Synthesis and Properties of a Novel Colorimetric and Fluorescent Turn-On Sensor for Cyanide

设计并合成一种具有席夫碱结构的新型荧光增强型探针7-(二丁基氨基)-2'-[(2-羟基苯亚甲基)氨基]-3-甲基-1-苯基-1H-螺[色烯并[2,3-c]吡唑-4,1'-异吲哚啉]-3'-酮(SCPz-S),通过(~1H NMR、^(13)C NMR及高分辨质谱(HRMS)对其结构进行了表征.利用紫外-可见光谱和荧光光谱考察探针SCPz-S的离子识别性能,研究发现探针SCPz-S在THF/H_2O(V∶V=1930∶50,5 mmol/L Tris-HCl,pH=7.4)中对CN-具有比色和荧光上的双重响应.加入CN-后,探针SCPz-S的体系会由无色变为淡黄色,在575 nm荧光增强1060倍.探针SCPz-S拥有良好的抗干扰能力和很广的p H适用范围(pH=3~12).探针对CN-的检出限达到1.121×10^(-8) mol/L,并对其识别机理进行了研究.

A novel fluorescent ＂turn-on＂ probe 7-（dibutylamino）-2＇-（（2-hydroxybenzylidene）amino）-3-methyl-1-phenyl-1 H-spiro[chromeno[2,3-c]pyrazole-4,1＇-isoindolin]-3＇-one（SCPz-S） with Schiff base structure was designed and synthesized,and characterized by ~1 H NMR, （13）C NMR and high resolution mass spectrometry（HRMS）. The UV-Vis and fluorescence spectra were used to investigate the ions recognition capability of probe SCPz-S. It was excavated that the probe SCPz-S has a dual colorimetric and fluorescence response to CN-in THF/H_2O（V∶V=1930∶50, 5 mmol/L Tris-HCl, pH=7.4）. After adding CN-, the system of probe SCPz-S will change from colorless to yellow, and fluorescence enhance 1060-fold at 575 nm. The sensor SCPz-S also has good anti-interference ability and a wide p H range from 3 to 12 applicable. Its LOD for CN-is 1.121×10-8 mol/L, and the recognition mechanism has been investigated.