佛山地区男性尖锐湿疣患者HPV感染现状及基因型分布

Prevalence and genotype distribution of HPV infection in male patients with condyloma acuminatum in Foshan region

目的探讨佛山地区男性尖锐湿疣患者中人乳头瘤状病毒(HPV)的感染情况、基因型别及其临床意义。方法选择500例临床诊断为尖锐湿疣的男性患者,从送检样本中提取病毒基因组DNA,通过聚合酶链式反应对病毒DNA进行扩增后,利用基因芯片对扩增产物进行导流杂交和分型检测。使用Excel对数据进行整理,并用SPSS 16.0统计软件对数据进行统计学分析。结果 500例尖锐湿疣男性患者中有388例检出HPV DNA阳性,其检出率为77.6%(388/500)。感染人群主要集中在21~40岁年龄段,占55.8%(279/500)。检出HPV阳性的患者中,低危型HPV感染291例,检出率为58.2%(291/500);高危型HPV感染仅有16例,检出率为3.2%(16/500);混合型HPV感染81例,检出率为16.2%(81/500)。单一型别感染291例,检出率为58.2%(291/500);多重感染97例,检出率为19.4%(97/500)。HPV单一感染患者中,感染亚型主要是HPV 6型,HPV多重感染以二重感染和混合型感染为主。结论佛山地区男性尖锐湿疣患者HPV感染以感染低危型HPV 6型为主,好发年龄段主要集中在21~40岁;单一感染居多,多重感染则以混合型二重感染为主;且HPV感染引起的尖锐湿疣具有一定的地域性差异。

Objective To investigate the infection status of human papillomavirus（HPV） genotype and its clinicalsignificance in male patients with condyloma acuminatum in Foshan region.Methods A total of 500 male patients diagnosedas condyloma acuminatum were chosen. Genomic DNA of virus was extracted from the submission of samples. Polymerasechain reaction（PCR） was used to amplify the virus DNA. The PCR products were hybridized and classified by the gene chip.Results Totally 388 HPV DNA positives were detected from the 500 male patients with condyloma acuminatum, with adetection rate of 77.6%（388/500）. The infection group was found mainly in the age group of 21-40 years old, accounting for55.8%（279/500）. Among the patients with HPV positive, there were 291 cases of low risk HPV infection, and the detection ratewas 58.2%（291/500）; there were 16 cases of high-risk HPV infection, and the detection rate was 3.2%（16/500）; there were 81 cases of mixed type HPV infection, and the detection rate was 16.2%（81/500）. There were 291 cases of single type infectionwith the detection rate of 58.2%（291/500）, and 97 cases of multiple infection with the detection rate of 19.4%（97/500）. In thecases of HPV single subtype infection, HPV 6 was the main infection subtype. In the cases of HPV multiple subtype infection,the main types were superinfection and mixed infection. Conclusions The male patients with condyloma acuminatum inFoshan region are mainly infected with low risk type HPV（HPV type 6）, which are mainly in the stage of 21-40 years old. Theyare mainly infected with single subtype infection. The multiple infection are mainly caused by mixed superinfection.HPVinfection caused by condyloma acuminatum has a certain regional difference.