Soleris微生物实时光电法与平板计数法测定生乳中菌落总数的比较研究

Comparison of Soleris Real-Time Optoelectronic System with Aerobic Plate Count Method for Detection of Total Bacterial Count in Raw Milk

建立Soleris微生物实时光电检测法检测生乳中菌落总数的快速测定方法,并将该方法与国标的平板计数法进行比较。结果表明:Soleris法检测生乳中菌落总数的标准曲线为y=-0.651 5x+8.263 9(y为菌落总数(lg(CFU/mL)),x为胶体栓变色时间(h)),相关系数R^2=-0.939 6,表明标准曲线相关性良好;重复性实验结果的相对标准偏差均小于5%;Soleris法与平板计数法测定结果的比较表明,2种方法之间没有显著性差异(P>0.05)。在本研究的实验条件下,Soleris法检测生乳中菌落总数的时间为0.9～11.8 h,与平板计数法的48 h相比大大缩短;另外,Soleris法操作简单、快捷,可同时检测128个样品,是一种高效、稳定的生乳中菌落总数快速检测的方法。

A method using a Soleris real-time optoelectronic microbial detection system was developed for the rapid detemaination of the total bacterial count in raw milk and compared with the conventional aerobic plate count method （APC） in this paper. The standard curve equation of the Soleris method was established as follows： y = -0.651 5x ＋ 8.263 9, where y is total bacterial count （lg （CFU/mL）） and x is detection time （h） （R2 =- 0.939 6） and it was verified to have good correlation. Repeatability tests showed relative standard deviation （RSD） values less than 5%. No statistically significant difference was observed comparing the two methods （P 〉 0.05）. Under the experimental conditions in this study, it took 0.9-11.8 h to analyze one sample using the Soleris method, which was greatly shortened compared with that （48 h） using APC. Furthermore, the Soleris method was simple and rapid and allowed simultaneous detection of 128 samples. Accordingly, it could be considered as an efficient and stable method for the rapid determination of the total bacterial count in raw milk.