摘要
冷冻超分辨光电融合成像技术近年来发展迅速,该技术结合了荧光显微镜特异性标记与冷冻电镜超高分辨率的优势,成为细胞原位结构研究的新手段,有望发展成为下一代成像技术.本文从发展背景、应用领域等几个方面,介绍了冷冻超分辨光电融合成像技术的概况及未来发展前景.
Cryogenic super-resolution correlative light and electron microscopy（cs CLEM）has been rapidly developed in recent years. This imaging methodology combines the specific labeling of fluorescence microscopy and the ultra-high resolution of electron microscopy, providing a new tool for resolving cellular structures in situ. It has the potential to become the next generation of imaging method. In this article, cs CLEM was reviewd based on its historical background, application fields, development prospects, etc.
作者
李尉兴
谷陆生
徐晓君
薛艳红
纪伟
徐涛
LI Wei-Xing;GU Lu-Sheng;XU Xiao-Jun;XUE Yan-Hong;JI Wei;XU Tao(National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomocromolecules,Institute of Biophysics Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Center far Biological Instrument Development,Core Facility far Protein Research,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China)
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2018年第9期957-960,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金重大仪器研制项目(31127901)资助~~
关键词
超分辨成像
冷冻电镜
光电融合
原位
super-resolution imaging
cryo electron microscopy
correlative light and electron microscopy
in situ
