奈必洛尔对非对称性二甲基精氨酸诱导损伤人脐静脉内皮细胞的保护作用研究

Protective Effect of Nebivolol on the Asymmetrical Dimethylarginine-induced Injury of Human Umbilical Vein Endothelial Cells

目的观察奈必洛尔对非对称性二甲基精氨酸(ADMA)诱导损伤人脐静脉内皮细胞(HUVECs)的保护作用。方法用ADMA16μmol/L培养HUVECs 24 h,不加或加入阿替洛尔20μmol/L、奈比洛尔(5μmol/L、10μmol/L、20μmol/L)预处理1 h。0.25%胰酶消化细胞,获取细胞悬液,离心收集细胞上清液,检测一氧化氮(NO)水平、一氧化氮合酶(NOS)活性。提取HUVECs总RNA,用RT-PCR分析内皮型一氧化氮合酶(eNOS)mRNA表达。结果 ADMA16μmol/L刺激HUVECs 24 h后,上清液NO含量和NOS活性降低,eNOS mRNA表达下调。奈比洛尔可剂量依赖性抑制ADMA所致的上述损伤。阿替洛尔对ADMA诱导的损伤无显著改善。结论奈必洛尔可保护ADMA诱导损伤HUVECs。

Objective To investigate the protective effect of nebivolol on asymmetrical dimethylarginine （ADMA） induced injury of hu -man umbilical vein endothelial cells （HUVECs）.Methods HUVECs were cultured with ADMA 16μmol/L for 24 hours in the absence orpresence of nebivolol （5μmol/L,10μmol/L or 20μmol/L） or atenolol （20μmol/L） for 1 hour.The supernaent in the conditioned mediumwas collected by centrifugation for determination of nitric oxide （NO） and nitric oxide synthase （NOS） activities.The expression of en -dothelial nitric oxide synthase （eNOS） mRNA was measured by real time polymerase chain reaction （RT PCR）.Results After theculture of HUVECs with ADMA 16μmol/L for 24 hours,the NO content and NOS activity in the supernatant decreased,and the expres -sion of eNOS mRNA was down regulated.Pretreatment with nebivolol concentration dependently attenuated the ADMA inducedinjury.Atenolol had no influence on the effects induced by ADMA.Conclusion Nebivolol protects the ADMA induced injury of HU -VECs