运载β-胡萝卜素的Pickering乳液的制备及表征

Preparation and Characterization of Pickering Emulsions Loaded with β-Carotene

为改善口．胡萝卜素的水相溶解性及生物利用率，采用淀粉纳米晶（SNC）为颗粒乳化剂稳定Pickering乳液，对β-胡萝卜素进行乳化包埋。考察了油相体积分数和卢．胡萝卜素含量对运载β-胡萝卜素的Pickering乳液储藏稳定性的影响。结果显示，当SNC含量为1％（以水相质量为基准），油相（溶有伊胡萝卜素的辛酸／癸酸甘油三酯）体积分数为50％，β-胡萝卜素含量为0．050％（以油相的质量为基准）时，采用含量为0．80％（以水相的质量为基准）的季铵盐壳聚糖（QCS）对Pickering乳液进行修饰后，乳液的乳析现象可明显改善，且储藏稳定性显著提高。体外模拟消化实验结果显示，QCS修饰后乳液中油脂的消化程度由56．68％±1．56％提高到71．70％±2．13％，β-胡萝卜素的生物可给率由33．59％±0．82％提高到57．62％±1．58％。采用QCS进行修饰不仅可以提高β-胡萝卜素Pickering乳液的稳定性，还可以提高乳液中油脂的消化程度和β-胡萝卜素的生物可给率。

In order to improve the solubility and bioavailability of β-carotene, the Pickering emulsions prepared with starch nanocrystals （SNC） as particle emulsifier were used to emulsify and encapsulate β-carotene. The factors affecting the storage stability of Pickering emulsion carrying β-carotene, such as volume fraction of oil phase and mass fraction of β-carotene were investigated. The results showed that when the content of SNC was 1% （based on the mass of aqueous phase）, the volume fraction of oil phase （caprylic/capric triglyceride containing β-carotene） was 50%, the content of β-carotene was 0.050% （based on the mass of oil phase）, the Pickering emulsion was modified with quaternary ammonium salt chitosan （QCS） at a content of 0.80% （based on the mass of aqueous phase）. The resultant Pickering emulsion exhibited an obvious improvement in separation of emulsion and a significant increase in storage stability. At the same time, the in vitro simulated digestion experiment showed that the digestion degree of the oil in the emulsion after QCS modification was increased from 56.68%±1.56% to 71.70%±2.13%, and the bioaccessibility of β-carotene was also increased from 33.59%±0.82% to 57.62%±1.58%. Therefore, QCS modification can not only improve the stability of β-carotene Pickering emulsion, but also improve the digestion degree of oil in the emulsion and the bioaccessibility of β-carotene.