自体脂肪间充质干细胞对大鼠肾冷缺血再灌注损伤的保护作用

Protective effect of autologous adipose derived stem cells in renal cold ischemia and reperfusion injury

目的观察大鼠自体脂肪问充质干细胞（ADSCs）移植对肾冷缺血再灌注损伤（IRI）的保护作用。方法将24只成年雄性Wistar大鼠随机分为3组：A组（假手术组）、B组（肾冷IRI组）、C组（肾冷IRI＋ADSCs组）：冷IRI即刻肾内注射剂量为2×10^6个的自体ADSCs，冷IRI后6h经阴茎静脉注射相同剂量自体ADSCs。再灌注24h处死大鼠观察。结果C组再灌注24h血清肌酐水平[（112．1±14．1）μmol／L]与B组[（169．5±17．5）μmol／L]比较显著降低，差异有统计学意义（P=0．001）；光镜下观察C组的肾脏组织病理学改变较B组明显减轻；反转录-聚合酶链反应（RT-PCR）分析显示C组肾组织FasmRNA表达水平（0．93±0．05）较B组（1．04±0．08）显著降低，差异有统计学意义（P=0．012），C组肾组织血红素氧合酶-1（HO-1）mRNA表达水平（0．35±0．03）较B组（0．244±0．05）显著增高，差异有统计学意义（P=0．001）。结论自体ADSCs移植通过抑制肾细胞凋亡及减轻氧化应激来保护大鼠肾冷IRI。

Objective To investigate the protective effects of autologous adipose derived stem cells （ADSCs） on rat renal cold ischemia and reperfusion injury （IRI）. Methods Adult male Wistar rats were equally randomized into group A （sham group）, group B （cold IRI group）, and group C （cold IRI plus immediate intra - renal administration of 2×10^6 autologous ADSCs, followed by intravenous autologous ADSCs at 6 hours post - reperfusion）. All rats were sacrificed at 24 hours after cold IR1 procedure. Re- sults Serum creatinine levels were significantly lower in group C [ （ 112.1±14. 1 ） μmol/L] than in group B [ （ 169. 5 ± 17.5 ） μmol/L, P = 0. 001 ]. The renal histopathologic morphology was attenuated in group C, as compared with group B. Furthermore, the mRNA expression levels of renal tissues Fas were significantly decreased in group C （0. 93 ± 0. 05）, and the mRNA expression levels of renal tissues heme oxgenase - 1 （ HO - 1 ） were significantly increased in group C （0. 35 ± 0.03 ） when compared with that in group B （ 1.04±0. 08, 0. 24 ±0. 05, respectively） （P =0. 012, 0. 001, respectively）. Conclusion Au- tologous ADSCs transplantation could protect renal cold IRI by inhibiting cellular apoptosis and alleviating oxidative stress effects.