仙人掌果水溶性提取物对体外培养新生大鼠头盖骨成骨细胞增殖、分化和矿化功能的影响

Effects of water - soluble extract from cactus pear fruit on proliferation, differentiation, and mineralization of new bone rat osteoblasts

目的观察仙人掌果水溶性提取物（CPFWE）对新生大鼠头盖骨成骨细胞增殖分化矿化功能的影响。方法制备新生大鼠头盖骨成骨细胞，噻唑蓝（MTF）法评价细胞生长增殖作用、分光光度计法测定碱性磷酸酶（ALP）活性与羟脯氨酸（Hyp）含量、茜素红染色法观察骨结节形成作用。结果中期分化实验研究显示，在50．000μg／ml的CPFWE刺激作用成骨细胞9d后，ALP活性可显著升高至（57．748±4．071）％（P=0．000）。同时，25．000μg／mlCPFWE作用22d后，Hyp含量提升到（26．444±0．990）％。在细胞外基质矿化期，50．000μg/mlCPFWE作用18d增加细胞矿化面积达（43．840±1．200）％，而6．250μg／mlCPFWE作用18d则增加细胞累积吸光度值为（48．241±1．760）％。结论CPFWE能显著地影响新生大鼠头盖骨成骨细胞中期分化和晚期矿化功能。

Objective To investigate the effects of the water - soluble extract from cactus pear fruit （CPFWE） on differentiation of obsteoblasts. Methods Rat osteoblasts were isolated as an in vitro model. MTY assay was used to evaluate the growth and proliferation of rat osteoblasts. The spectrophotome- ter was used to determine alkaline phosphatase （ALP） activity and hydroxyproline （Hyp） content. By aliz- arin red staining, bone nodule formation was observed. Results In the middle stage of differentiation, CPFWE observably stimulated ALP activity and Hyp content in osteoblasts. Especially, CPFWE increased the ALP activity by （57. 748 ±4. 071 ） % at the concentration of 50. 000 μg/ml for 9 days and Hyp content by （26. 444 ± 0. 990） % at the concentration of 25. 000 μg/ml for 22 days as compared with the control group. In the later stage, the CPFWE significantly increased the area of nodules by （43. 840 ± 1. 200 ） % at 50. 000 μg/ml concentration and increased IOD by （48. 241 ± 1. 760）% at 6. 250 μg/ml for 18 days. Conclusion The effects of CPFWE on bone formation by rat osteoblasts suggest that these phenolies may have beneficial effects on bone health.