质谱法检测食源性致病菌技术研究进展

Advances in Testing Foodborne Pathogenic Bacteria by Mass Spectrometry

食源性致病菌存在广泛,能够引起人类的疾病甚至死亡,研究发现超过一半的食品安全问题来源于食源性致病菌的污染。如何快速有效地检测出食源性致病菌是预防和控制食品安全问题的关键环节。系统地介绍了检测食源性致病菌的方法,包括传统培养法、代谢学法、分子生物学法、免疫学方法等传统方法以及新兴的质谱法。质谱法有检测效率高、操作简便、灵敏度高等优点,着重对质谱法的原理、应用以及未来的发展趋势进行了阐述,以期为该技术的研究开发和推广应用提供参考。

Foodborne pathogens widely exist and can cause human disease and even death. The study has found that more than half of food safety problems stem from the contamination of foodborne pathogens. Therefore,how to detect food-borne pathogens rapidly and effectively is a key link in the preventing and controlling food safety. This paper systematically reviewed the methods of detecting foodborne pathogens,including traditional methods such as traditional culture method,metabolic method,molecular biological method and immunological method,and the new mass spectrometry method. Since the mass spectrometry has the advantages of high detection efficiency,simple to operate,and high sensitivity,hence,this paper focuses on the principle,application and future development trend of mass spectrometry,hopefully to provide reference for the research and development and popularized the application of the technology.