胃癌组织HIF-1α和HIF-2α表达及临床病理意义探讨

Expression of HIF-1α and HIF-2α in gastric carcinoma tissues and their clinico pathologic significance

目的缺氧是恶性肿瘤普遍存在的现象,缺氧直接导致一系列基因和蛋白的表达,如缺氧诱导因子(hypoxiainducible factors,HIFs),是促使肿瘤发生浸润、转移的根本原因。本研究探讨缺氧诱导因子HIF-1α与HIF-2α在胃癌组织中的表达,并探讨其与临床病理特征的相关性。方法选取2015-01-01-2016-06-01靖江市中医院40例胃癌患者胃癌组织样本作为实验组,同期40例非胃癌患者胃镜活检下慢性胃炎组织作为对照组。采用免疫组化SP法分别检测样本中HIF-1α与HIF-2α表达。结果实验组HIF-1α阳性表达率为70.0%(28/40),HIF-2α阳性表达率为40.0%(16/40),与对照组(10%,5%)比较差异有统计学意义,P<0.05。HIF-1α在胃癌标本的表达与肿瘤TNM分期及有无淋巴结转移相关,差异有统计学意义,均P<0.05;与性别、年龄、肿瘤分化程度无关,均P>0.05。HIF-2α在胃癌标本的表达与肿瘤TNM分期及有无淋巴结转移相关,差异有统计学意义,均P<0.05;与性别、年龄、肿瘤分化程度无关,均P>0.05。结论 HIF-1α与HIF-2α的高表达与胃癌发生和进展关系密切,与TNM分期密切相关,有望成为胃癌诊断中新型的判断肿瘤侵袭和转移的生物学指标。

OBJECTIVE Hypoxia is a ubiquitous phenomenon in malignant tumors,which causes a series of expres- sion of gene and protein risen, such as hypoxia-inducible factors which is essential reason driven tumor invasion and metas- tasis. The aim of this study was to investigate the expression of HIF-1α and HIF-2α and explore their relation to clinico- pathologic characteristics and prognosis of gastric cancers. METHODS Immunohistochemical SP method was carried to detect the expression of HIF-1α and HIF-2α in 40 cases of gastric cancer,and 40 cases of tissue with gastritis for contral group respectively. All of the samples were from to Jingjiang Chinese Traditional Medicine Hospital from January 2015 to June 2016. RESULTS The positive expression rate of HIF-1α and HIF-2α in the gastric cancer was 70.0% and 40.0%, and the difference was statistically significant compared with the contral group（P〈0.05）. The expression of HIF-1α in gastric cancer was closely related to TNM stage and lymph node metastasis （both P〈0.05）. The expression of HIF-1α was not related to gender, age and tumor differential degree. The expression of HIF-2α in gastric cancer was closely related to TNM stage and lymph node metastasis（both P〈0.05）, and was not related to gender, age and tumor differential de- gree. CONCLUSIONS The positive expression of HIF-lα and HIF-2α is closely related to the tumorigenesis and progress of gastric cancer,and their expression is closely related to TNM stage. HIF-lα and HIF-2α can be useful as biological indi- cators to predict the invasion and metastasis of gastric cancer.