IL-10启动子区-1082基因多态性与脓毒血症易感性的关系研究

Study on the relationship between locus 1082 gene polymorphism in IL-10 promoter region and susceptibility to sepsis

目的分析脓毒血症患者IL-10的启动子区域转录起始位点上游第1082位点的多态性,探讨其与脓毒血症易感性之间的关系。方法采用聚合酶链反应-限制性片段多态性(PCR-RELP)检测IL-10基因-1082位点多态性,SPSS17.0分析各位点的基因型分布和等位基因频率。结果 IL-10-1082位点的多态性在脓毒血症组(AA:58.16%、GA:39.80%、GG:2.04%)与对照组(AA:73.75%、GA:26.25%)的基因频率有显著性差异(P<0.05)。脓毒血症组中GA基因型的频率显著高于对照组(P=0.038),脓毒血症组中G等位基因的频率显著高于对照组(P=0.015)。结论 IL-10-1082 GA基因型携带者比AA基因型患者的脓毒血症风险性高,IL-10-1082的G等位基因比A等位基因患脓毒血症的风险性高。

Objective To analyze the polymorphism at locus 1082 in the upstream of the transcriptional start site in the promoter region of IL-10 in sepsis patients, and to explore its relationship with susceptibility to sepsis. Methods Polymerase chain reaction-restriction fragment polymorphism（PCR-RELP）was used to detect the IL-10-1082 polymorphism. SPSS 17.0 was used to analyze the genotypic distribution and allele frequencies at each locus. Results The gene frequency of polymorphism of IL-10-1082 in the sepsis group （AA： 58.16%, GA： 39.80%, GG： 2.04%） and the control group（AA： 73.75%, GA： 26.25%） had significant differences（P〈0.05）. The frequency of GA genotypes in the sepsis group was significantly higher than that in the control group（P=0.038）. The frequency of G allele in the sepsis group was significantly higher than that in the control group（P=0.015）. Conclusion IL-10 -1082 GA genotype carriers have a higher risk of sepsis than those with AA genotypes. The G-10 allele of IL-10-1082 has a higher risk of sepsis than the A allele.