MGMT及表皮生长因子受体在喉鳞癌中的表达及对预后的影响

Expression of MGMT and epidermal growth factor receptor in laryngeal squamous cell carcinoma and its effect on prognosis

目的研究探讨喉鳞状细胞癌中MGMT及表皮生长因子受体蛋白的表达与临床资料间的关系及其对治疗预后的影响。方法标本均选取2016年1月~2017年6月于我院耳鼻喉科手术治疗的93例喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)患者的石蜡切片,经病理学确诊为LSCC标本。选取20例健康喉黏膜组织标本作为对照组。采用免疫组化染色法检测O6-甲基鸟嘌呤-DNA-甲基转移酶(O6-methylguanine-DNA-methyltransferase,MGMT)与表皮生长因子受体蛋白(epidermal growth factor receptor,EGFR)的表达水平。评价肿瘤组与对照组之间MGMT、表皮生长因子受体蛋白(EGFR)的表达差异;评价肿瘤组中MGMT、EGFR的表达水平与其各项临床资料间的关系;评价肿瘤组中MGMT与EGFR表达之间的关系。结果肿瘤组与对照组之间MGMT、EGFR的表达比较,差异具有统计学意义;肿瘤组中MGMT、EGFR的表达水平与病理分期和分化程度呈正相关关系;肿瘤组中MGMT与EGFR表达呈正相关。结论本次研究证明喉鳞状细胞癌中MGMT、EGFR的表达与病理分期和分化程度呈正相关,MGMT、EGFR的高表达往往意味着肿瘤预后不良且比通过疾病分期预后更为准确。MGMT、EGFR的表达作为LSCC的生物指标值得临床推广。

Objective To investigate the relationship between the expression of MGMT and epidermal growth factor receptor （EGFR） and clinical data in laryngeal squamous cell carcinoma and its effect on the prognosis of treatment. Methods Paraffin section of 93 cases of Laryngeal squamous cell carcinoma（LSCC） who were treated in the Department of Otolaryngology in our hospital from January 2016 to June 2017 were selected. All the specimens were pathologically confirmed as LSCC specimens. 20 specimen of healthy laryngeal mucosa were selected as control group. The expression of O6-methylguanine-DNA-methyhransferase （MGMT） and epidermal growth factor receptor（EGFR） was detected by immunohistoehemieal staining. The differences in expression of MGMT and epidermal growth factor reeeptor（EGFR） protein between tumor group and control group were evaluated. The relationship between MGMT, EGFR expression levels and their clinical data in tumor groups was evaluated. The relationship between MGMT and EGFR expression in tumor groups was evaluated. Results There were significant differences in the expression of MGMT and EGFR between the tumor group and the control group （P〈0.05）. The expression levels of MGMT and EGFR in the tumor group were positively correlated with pathological stage and differentiation （P〈0.05）. There was a positive correlation between MGMT and EGFR expression in the tumor group（P〈0.05）. Conclusion This study demonstrates that the expression of MGMT and EGFR is positively correlated to the pathological stage, differentiation in laryngeal squamous cell carcinoma. The high expression of MGMT and EGFR often means that the prognosis of the tumor is poor and it is more accurate in prognosis than the staging of the disease. The expression of MGMT and EGFR is worthy of clinical promotion as a biological indicator of LSCC.