上海男男性行为人群HIV抗体唾液快检试剂现场使用效果评价

Oral mucosal transudate rapid test kit for HIV antibody screening among men who have sex with men in Shanghai city: a field study

目的对一种国产口腔粘膜渗出液艾滋病病毒(HIV 1/2型)抗体检测试剂进行现场实验研究,评价其敏感性、特异性、功效率、阳性预测值和阴性预测试值。方法在同志酒吧、自愿咨询检测门诊和艾滋病患者中采集391名男男性行为人群(MSM)的口腔粘膜渗出液和血液样本,分别使用血液HIV抗体检测试剂和口腔粘膜HIV 1/2型抗体检测试剂进行现场检测,对比2者的检测结果。结果口腔粘膜HIV 1/2型抗体检测试剂敏感性为93.52%,特异性为99.65%,功效率为97.95%,阳性预测值为96.43%,阴性预测值为98.95%。结论该口腔粘膜HIV 1/2型抗体检测试剂具有很高的流行病学预测价值,适合在酒吧、会所、浴室、公园等现场对MSM人群广泛开展筛查。

Objective To carry out a field study on an oral mucosa transudation（OMT） test kit for human immunodeficiency virus type 1 and type 2（HIV-1/2） antibody detection among men who have sex with men（MSM） and to evaluate its sensitivity, specificity, efficiency, positive predictive value and negative predictive value. Methods Oral mucosal transudate specimen and serum samples were collected among 391 MSM recruited at gay bar, voluntary counseling test（VCT） clinics; then an on-site detection for HIV-1/2 antibody with the OMT test kit and blood rapid test kit was conducted. The differences in detection results of the two methods were compared. Results The detection results of OMT test kit demonstated a sensitivity of 93.52%, a specificity of 99.65%, an efficiency of 97.95%, a positive predictive value of96.43%, and a negative predictive value of 98.95%, respectively. Conclusion The OMT test kit is of high predictive value for HIV-1/2 antibody sceening and is appropriate for wide utilization in on-site screening of HIV infection among MSM at gay bar, club, bath and garden.