HPLC定量分析千斤拔药材中染料木苷和染料木素的含量

Quantitative Determination of Genistin and Genistein in Moghania Medicinal Material by HPLC

目的:建立千斤拔药材的质量控制方法。方法:采用高效液相色谱法测定其中的染料木苷和染料木素的含量;色谱柱为Kromasil 100-5 C18(250 mm×4.6 mm,5μm),梯度洗脱,流动相为乙腈-0.1%磷酸水溶液,流速为1 m L·min-1,检测波长为260 nm。结果:染料木苷和染料木素分别在质量浓度17.483~279.720μg·m L-1和4.088~65.406μg·m L-1与峰面积线性关系良好。平均回收率,染料木苷为104.27%,RSD为2.61%(n=9);染料木素为97.16%,RSD为5.54%(n=9)。不同产地千斤拔药材中染料木苷和染料木素的含量有明显差异。结论:建立的含量测定方法操作简便、分离效果好、准确,能满足千斤拔药材中染料木苷和染料木素含量的准确测定,为千斤拔药材的质量评价以及合理开发利用提供了科学依据。

Objective： To establish a method for the quality control of Moghania medicinal material. Methods： An HPLC method for quantitative analysis of genistin and genistein in the Moghania medicinal material,including the roots of Moghania macrophylla（Willd.） O. Kuntze and M. philippinensis（Merr. et Rolfe） Li,was carried out. The method was used by HPLC with Kromasil 100-5 C18 column（250 mm × 4. 6 mm,5 μm） using gradient elution with acetonitrile and 0. 1% aqueous phosphoric acid as the mobile phase at a flow rate of 1 m L·min^-1,and the detection wavelength was 260 nm. Results： Genistin and genistein showed good linearity in the ranges of 17. 483-279. 720 μg·m L-1 and 4. 088-65. 406 μg·m L^-1,respectively. The average recoveries were 104. 27% with RSD 2. 61%（n = 9） for genistin and 97. 16% with RSD 5. 54%（n = 9） for genistein. The contents of genistin and genistein in Moghania medicinal material from different habitants showed remarkable difference. Conclusion： The developed method is simple,well efficiency and precise,which can be used to determine content of genistin and genistein in Moghania medicinal material and provided the scientific basis for the quality evaluation as well as rational development and utilization of Moghania medicinal material.