基于指纹图谱与聚类分析评价不同产地白鲜皮药材质量

Clustering Analysis on HPLC Fingerprint Characteristics of Dictamnus dasycarpus from Different Areas

目的:考察不同产地白鲜皮药材质量,为白鲜皮药材质量控制提供参考。方法:采用HPLC法,以乙腈-水为流动相进行梯度洗脱,在210~500 nm检测,提取228 nm波长处的色谱图计算相似度,以白鲜碱峰为参照峰,计算相对峰面积,进行聚类分析。结果:15批次白鲜皮药材的相似度均大于0.9,确定13个共有峰,指认出白鲜碱、黄柏酮、梣酮3个峰,聚类分成3类。结论:该方法为白鲜皮药材质量全面评价体系的建立提供了参考。

Objective： To evaluate the quality of root cortex of Dictamnus dasycarpus from different areas. Methods： The HPLC fingerprint of root cortex of D. dasycarpus were eluted with the mobile phase consisted of acetonitrile and water in gradient mode. The flow rate was 1. 0 m L·min^-1 and the detection wavelength was 228 nm. Hierarchical cluster analysis was applied. Results： Thirteen common peaks were selected in the standard fingerprint and the similarity was over 0. 9. The root cortex of D. dasycarpus from 15 different batches could be divided into three grades through the results of hierarchical cluster analysis. Conclusion： The fingerprint study and hierarchical cluster analysis method can be applied for quality assessment of the root cortex of D. dasycarpus.