薄层扫描法同时测定三七细粉中皂苷含量

Content Determination of Ginseng Saponin by TLC-scanning in Panax Notoginseng Powder

目的:建立同时测定三七细粉中人参皂苷Rb1、三七皂苷R1和人参皂苷Rg1含量的方法。方法:采用双波长薄层扫描法,吸附剂为硅胶G,展开剂为三氯甲烷-甲醇-水(13∶7∶2)10℃以下放置12 h的下层液,显色剂为10%硫酸乙醇溶液,110℃加热显色,测定波长510 nm,参比波长700 nm。结果:三种皂苷分别在0.504~5.04、0.501~5.01、0.492~4.92μg范围呈良好的线性关系;平均回收率分别为96.40%、96.97%、98.97%,RSD分别为1.40%、0.98%、1.70%(n=6)。结论:该方法操作简便、快速、高效、准确。

Objective： To establish a method for determination of ginsenosides content in Panax notoginseng powder. Methods： The double wavelength thin layer scanning method was applied with silica gel G as the adsorbent,the low layer of chloroform-methanol-water（13： 7： 2） under 10 ℃ placed 12 h was used as developing solvent,10% ethanol solution of sulfuric acid was used as the chromogenic agent with 110 ℃ heating,the determining wavelength was set at 510 nm,reference wavelength was 700 nm. Results： The three saponins showed a good linear relationship in the range of 0. 504-5. 04,0. 501-5. 01,0. 492-4. 92 μg. The average recoveries were 96. 40%,96. 77%,98. 97%; RSD were 1. 40%,0. 98%,1. 70%（n = 6）. Conclusion： The established method is simple,rapid and accurate.