地方特色蛋鸡配套系亲本群中不同亚群禽白血病病毒的混合感染

Mixed Infection of Different Subgroups of Avian Leucosis Virus in Parent Groups of Local Laying Hens

采用ELISA、病毒分离和多聚酶链式反应(PCR)方法对江苏某地方特色蛋鸡亲本群的禽白血病病毒(ALV)p27抗原、ALV-A/B和ALV-J抗体以及病原核酸进行了检测,结果显示该配套系母本群泄殖腔拭子p27抗原阳性率为40.6%,ALV-A/B抗体阳性率为3.8%;父本群泄殖腔p27抗原阳性率为24.4%,ALV-J抗体阳性率为19.4%;细胞培养物IFA检测显示父本分离株JS12JD03能与J亚群特异单克隆抗体Je-9反应,在荧光显微镜下产生绿色荧光,而母本分离株JS12JD01和JS12JD02则无;gp85氨基酸序列同源性分析显示母本鸡群分离株JS12JD01属于A亚群、JS12JD02属于B亚群,父本鸡群分离株JS12JD03属于J亚群ALV,提示了该地方特色蛋鸡亲本群存在A、B、J亚群ALV的混合感染。

Avian leucosis virus（ALV） infection was investigated with ELISA test, DF1 cell culture and PCR in local Parent flocks. The result of ELISA test showed that the positive rate of p27 antigen was 40.6%, the positive rate of antibody against subgroups A/B was 3.8% in female parent flocks. While in male parent flocks, the positive rate of p27 antigen was 24.4%, the positive rate of antibody against subgroups J was 19.4%. By IFA detection,we found only JS12 JD03 isolated from male parent flocks can combine with monoclonal antibody Je-9, produced Green fluorescence under the fluorescence microscope, while the JS12 JD01 and JS12 JD02 isolated form femal parent flocks did not. Then by PCR amplification of env gene. Comparison of gp85 amino acid sequences between the isolation strains and the other ALV strains from different subgroups data in Gen Bank indicated that JS12 JD01 had the higher homology with reference strains of subgroup A. JS12 JD02 had the higher homology with reference strains of subgroup B. JS12 JD03 had the higher homology with reference strains of subgroup J. All the observations indicate that there is coinfection of subgroup A, subgroup B and subgroup J ALV infection in this parent groups of local laying hens.