摘要
目的:探讨补骨脂对氢化可的松所致肾阳虚模型动物燥毒性影响的主要提取部位。方法:以脏器指数、肝肾功能、血清cAMP/cGMP、TNF-α、Na^+-K^+-ATP酶含量为评价指标,观察补骨脂石油醚部位、乙酸乙酯部位、正丁醇部位、水部位对氢化可的松致肾阳虚模型大鼠的影响。结果:乙酸乙酯部位组较正常组、模型组对肝、肾、肾上腺、胸腺指数,TP、Alb、ALT、BUN均有显著性影响(P<0.05);正丁醇组较正常组、模型组对肾指数、TP、cAMP/cGMP、血清TNF-α和Na^+-K^+-ATP酶的含量均有显著性影响(P<0.05)。结论:补骨脂中产生燥毒性的物质主要存在于乙酸乙酯部位和正丁醇部位中。
Objective: To explore the effects of different extraction sites of Fructus Psoraleae on dry toxicity of hydrocortisone induced yang deficiency of kidney model. Methods: Taking organ index, liver and kidney function, serum cAMP/cGMP, TNF-α, Na^+-K^+-ATP ase content as evaluation index to observe effect of the extract of petroleum ether, ethyl acetate, n-butyl alcohol and water on rats with hydrocortisone induced yang deficiency of kidney. Results: The ethyl acetate extract of Fructus Psoraleae can significantly influence on the indexes of kidney, liver, adrenal gland, thymus, and TP, Alb, GPT, BUN(P〈0.05). While the N-butyl alcohol extract of Fructus Psoraleae can influence indexes of kidney, TP, and the content of cAMP/cGMP, Na^+-K^+-ATP enzyme in serum significantly(P〈0.05). Conclusion: Dry toxicity ingredients mainly exist in ethyl acetate extract and N-butyl alcohol extract of Fructus Psoraleae.
作者
夏亚楠
朱月刚
丁雷
毕胜
余凌英
XIA Ya-nan;ZHU Yue-gang;DING Lei;BI Sheng;YU Ling-ying(Anhui Puren Chinese Herbal Medicine Co.Ltd.,Bozhou 236800,China;engdu University of TCM,Chengdu 611137,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第9期4079-4081,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81303228)~~
关键词
补骨脂
不同提取部位
肾阳虚
肝肾功能
燥毒性
乙酸乙酯
正丁醇
Fructus Psoraleae
Different extraction sites
Yang deficiency of kidney
Liver and kidney function
Drytoxicity
Ethyl acetate
N-butyl alcohol