温度对中华鳖血浆皮质酮水平、免疫生化指标及抑菌效果的影响

Influences of temperature on plasma corticosterone levels,immunobiochemical indices in Chinese soft-shelled turtle Pelodiscus sinensis and antibacterial effects

对正常生长状态下3种月龄(12、18、24月龄)的中华鳖血浆皮质酮(corticosterone,CORT)进行了测定,并以(28±2)℃为恒温对照组,研究变温(10、20、33℃)对同一年龄段中华鳖血浆CORT水平、免疫生化指标以及抑菌效果的影响。结果表明:12月龄与24月龄个体间血浆CORT水平有显著性差异(P<0.05);各温度处理组中华鳖血浆CORT含量随时间呈先升后降的变化趋势,其中10℃和20℃组的CORT水平在4和6 h时与对照组有显著性差异(P<0.05);各温度处理组酶活性和蛋白含量随时间的增加先升后降,但与对照组无显著性差异(P>0.05);血浆抑菌效果与血浆CORT水平呈负相关。实验表明,温度骤变会促使中华鳖产生应激反应,并使机体血浆内的CORT水平增加,免疫生化发生改变,抑菌效果下降,从而降低机体的免疫力。

We measured the levels of plasma corticosterone（ CORT） from three samples of Pelodiscus sinensis（ 12,18 and 24 months after hatching） under normal growth condition. The effects of different temperatures（ 10,20 and 33 ℃） on CORT levels,immune biochemical indicators and antibacterial effects were examined,with（ 28 ± 2） ℃（ constant temperature） as control.The results showed a significant difference in CORT levels between 12-and 24-month-old individuals（ P〈0.05）. The CORT level in each group showed a tendency of first rising and then decreasing over time. The CORT level in the 10 ℃ and 20 ℃ groups was significantly different from the control after treated for four and six hours（ P〈0.05）. The enzyme activity and protein content of each temperature treatment first increased and then decreased over time. There was no significant difference compared to the control（ P〉0. 05）. The antibacterial effect of plasma was negatively correlated with CORT level. Our results indicated that sudden changes in temperature caused stress response for P. sinensis,which increased the CORT level,changed the immune biochemistry,and reduce the antibacterial effect,with a negative effect on organism immunity.