青海湖裸鲤肠道乳酸菌多样性与抗生素抗性的研究

Research of Lactobacillus diversity and antibiotic resistance in the intestine of Qinghai lake naked carp

通过分析裸鲤肠道的微生物多样性,进一步对分离出的乳酸菌代表菌株的形态学特征、生理生化特征进行分析,运用牛津杯双层平板法进行耐抗生素测定,通过16Sr RNA基因测序结果与blast基因数据库比对将代表株鉴定到种或者亚种水平。从中筛选出具有优秀抑菌活性及优秀抗逆性的乳酸菌代表株。为青海湖裸鲤肠道益生菌的开发和应用提供理论支持。

By analyzing the microbial diversity of nudibranch intestine, the morphological characteristics, physiological and biochemical char- acteristics of the isolates of the lactic acid bacteria isolates were further analyzed, antibiotic resistance determination was performed using the Oxford Cup double-layer plate method, 16S rRNA gene sequencing results and blast gene database alignment will identify the strain to spe- cies or subspecies level. Screening out the representative lactic acid bacteria with excellent antibacterial activity and excellent stress resistance. Provide theoretical support for the development and application of probiotics in Qinghai Lake.