均匀设计法优化红枣红色素的提取及其稳定性研究

Study on the Extraction and Stability of Red Jujube Red Pigment by Uniform Design Method

采用均匀设计法设计实验,优化了红枣红色素的最佳提取工艺条件,并研究其稳定性。通过U17(178)均匀设计方案得到了以色素提取率y为因变量,提取剂浓度x1、料液比x2、提取时间x3、提取温度x4为自变量的回归方程y=11.71465284+21.11594674x1-0.48066931x2-0.08376224x3+0.44222659x4。回归方程的R2=0.9937,各因素总体的P值都远小于0.05,说明模型拟合度高。实验结果表明:当NaOH的浓度为0.5mol/L,料液比为1∶25(g/mL),温度为60℃,提取时间为5h,提取级数为3级时,红枣红色素的提取率最高,为93.11%;40℃阴暗避光处,无抗坏血酸的环境是红枣红色素最佳稳定贮存条件。

In this paper,the optimum extraction conditions and stability of red jujube red pigment are optimized by uniform design experiment.Through U17（178）uniform design scheme,obtain the regression equation with the pigment extraction rate y as the dependent variable,the extracting agent concentration,the material-liquid ratio,the extraction time and the extraction temperature as the independent variables.The regression equation is y=11.71465284＋21.11594674 x1-0.48066931 x2-0.08376224 x3＋0.44222659 x4.The R2 of the regression equation reaches 0.9937,and the P value of all factors is far less than 0.05,indicating that the model fits well.The experimental results show that when the concentration of NaOH is 0.5 mol/L,the ratio of solid to liquid is 1∶25（g/mL）,the temperature is60 ℃,the extraction time is 5 h,the extraction stage is grade 3,the highest extraction rate of red pigment from red jujube is 93.11%.The best stable storage conditions of jujube red pigment is the dark shaded environment of 40 ℃ without ascorbic acid.