精氨酸加压素对大鼠下丘脑视前区AMPA受体GluR1-3蛋白表达的影响

Effect of arginine vasopressin on GluR1-3 protein expression of AMPA receptors in rat preoptic area of hypothalamus

目的研究精氨酸加压素(AVP)对大鼠下丘脑视前区(POA)α-氨基-3-羟基-5-甲基异恶唑-4-丙酸(AMPA)受体GluR1-3 mRNA和蛋白质表达的影响。方法腹腔注射AVP(10μg/kg)或AVP V1a受体抑制剂(30μg/kg)0.5 h后,用反转录实时荧光定量PCR和Western blot检测视前区AMPA受体亚基GluR1、GluR2和GluR3 mRNA和蛋白表达。结果与对照组相比,V1a受体抑制剂明显降低了GluR2 mRNA表达(P<0.05);AVP能显著上调视前区AMPA受体亚基GluR1和GluR2蛋白水平(P<0.01),减少GluR3蛋白表达(P<0.01),但V1a受体抑制剂不能阻断这一作用。结论外源性AVP主要通过影响AMPA受体亚基蛋白表达来调制大鼠视前区AMPA受体介导的谷氨酸能突触传递。

Objective To investigate the effect of arginine vasopressin （AVP） on the mRNA and protein expression of GluR1- 3 in the preoptic area of the rat hypothalamus. Methods After 0.5 h for intraperitoneal injection of AVP （10 μg/kg） or V1a receptor antagonist （30 μg/kg）, the changes of the mRNA and protein expression of POA-AMPA receptor GluR1, GluR2 and GluR3 subunits were analysed by reverse transcriptase real-time fluorescence quantitative PCR （RT-qPCR） and Western blot technology. Results Compared with control group, the GluR2 mRNA was significantly decreased by the injection of V1a receptor antagonist（ P 〈0.05）. AVP could up-regulate the protein level of POA GluR1,GluR2 receptor subunits（ P 〈0.01）, but down-regulate the expression of GluR3 protein （ P 〈0.01）. Nevertheless,the effect of AVP could not be blocked by V1a receptor antagonist. Conclusions Exogenous AVP is involved in modulation of glutamatergic synaptic transmission in the POA by regulation expression of AMPA receptor proteins.