复方当归茶的体外抗氧化和体内免疫调节活性

Antioxidant Activity in Vitro and Immunocompetence in Vivo of Compound Angelica sinensis Tea

研究复方当归茶(CAT)的体外抗氧化和体内免疫调节活性。制备不同浓度的CAT溶液,分别用钼蓝显色法、DPPH分析法、邻苯三酚自氧化法、水杨酸法对CAT进行总抗氧化活性、DPPH自由基、超氧阴离子自由基、羟自由基清除能力的检测;采用小鼠腹腔巨噬细胞吞噬鸡红细胞实验、血清溶血素水平测定实验、NK细胞活性测定和小鼠网状内皮系统吞噬功能实验研究CAT的免疫调节作用。结果表明:CAT具有一定的体外抗氧化活性,随着受试物浓度的增加,总抗氧化活性逐渐增大,CAT能明显清除DPPH自由基和·OH自由基,IC50分别为3.2226、20.8848 mg/m L,CAT对O-2·自由基具有较弱的清除作用;与模型组比较,CAT高、中剂量组小鼠腹腔巨噬细胞吞噬百分率和吞噬指数均有显著升高(p<0.05),CAT高剂量组小鼠血清溶血素抗体水平极显著升高(p<0.01),CAT高、中剂量组小鼠NK细胞活性均有显著升高(p<0.05),CAT高剂量组小鼠脾脏指数均有显著升高(p<0.05),CAT高、中剂量组K值和α值均显著或极显著升高(p<0.01,p<0.05),综合分析发现复方当归茶具有一定的体外抗氧化活性和体内免疫增强作用。

To explore the antioxidant in vitro and immunological activities of compound Angelica sinensis tea（ CAT）,the different concentrations of CAT were prepared,the total antioxidant activity,DPPH radical,superoxide anion radical determination,hydroxyl radical scavenging ability of CAT were measured respectively by molybdenum blue colorimetric method,DPPH analysis method,adjacent benzene three phenol autoxidation and salicylic acid method. The immunological activities of CAT were measured by peritoneal macrophages based on the red blood cells of chicken in mice,serum hemolysin level,and activity of NK cells and mice reticuloendothelial system.The results showed that CAT had certain antioxidant activity in vitro,with the increase of sample concentration,total antioxidant activity increased gradually,CAT was able to scavenge DPPH free radical and·OH free radical obviously,IC50 were 3.2226,20.8848 mg/mL,but weakly on scavenging O2^-· free radicals,peritoneal macrophage phagocytic percentage and phagocytic index were significantly higher in high and middle dose group（ p〈0.05）,serum hemolysin antibody levels were significantly increased in CAT high dose group（ p〈0.01）,NK cell activity in mice were significantly higher in high or middle dose group（ p〈0.05）,spleen index were significantly higher in high dose group（ p〈0.05）,K and α values were significantly increased in high and middle dose group（ p〈0.01,p〈0.05）.Comprehensive analysis found that CAT had certain antioxidant activity and non-specific immune enhancement effect.