摘要
目的:比较中药六味地黄汤与单味颗粒混合液有效成分的含量。方法:采用高效液相色谱法,以乙腈-磷酸水溶液为流动相进行梯度洗脱,流速为1.0 m L/min,检测波长238 nm。定量分析2种溶液中的已知有效活性成分马钱苷、丹皮酚、没食子酸、芍药苷的含量变化。结果:3批六味地黄传统汤剂种马钱苷、丹皮酚、没食子酸、芍药苷的含量明显高于同批的单味颗粒剂混合液中成分的含量,分别为3批单味颗粒的1.45、1.36、1.43倍。结论:中药六味地黄汤单味颗粒剂混合液低于六味地黄汤有效成分含量,存在明显差异,本研究为临床中药汤剂、单味颗粒剂合理应用提供科学依据。
Objective: To compare the content of the effective components in the mixture of Liuwei Dihuang Decoction and single ingredient mixture.Methods: The gradient elution was carried out by using high performance liquid chromatography with acetonitrile phosphoric acid aqueous solution as mobile phase.The flow rate was 1.0 mL/min and the detection wave length was 238 nm.Quantitative analysis of changes in the content of two kinds of solution of known active ingredients loganin,paeonol,gallic acid,and paeoniflorin.Results: The 3 batch of Liuwei Dihuang Decoction,loganin,paeonol,gallic acid,and paeoniflorin content was significantly higher than that of the single component content of the same batch of granules in a mixed solution,respectively,for the 3 batch of single granule 1.45,1.36,1.43 times.Conclusion The traditional Chinese medicine Liuwei Dihuang Decoction mixture of single ingredient is lower than the effective ingredient content of Liuwei Dihuang Decoction,with significant differences.This study provides a scientific basis for the clinical application of traditional Chinese medicine decoction and single granule.
作者
胡耶芳
林玲
易春霞
颜剑
黎剑
Hu Yefang;Lin Ling;Yi Chunxia;Yan Jian;Li Jian(Pharmacy Department,Guilin Hospital of Traditional Chinese Medicine,Guilin 541002,China)
出处
《世界中医药》
CAS
2018年第7期1756-1758,1762,共4页
World Chinese Medicine
基金
广西壮族自治区卫生和计划生育项目(Z2016007)