芽孢杆菌发酵炮制中药红花增强溶血栓药效研究

Study on the Efficacy of Bacillus Fermented Chinese Medicine Flos Carthami in Enhancing Thrombolytic Effect

目的:探讨芽孢杆菌发酵炮制中药红花增强溶血栓药效。方法:采用系统数值化和数值系统调控技术对发酵炮制条件进行合理适量优化,用角叉菜胶法对小鼠尾部进行了血栓实验,检测了大鼠纤溶和凝血指标。结果:在相同剂量下(1000 U/m L)A红发组(红花与C2-13共发酵组)比其余各组有明显缩短血栓长度(P<0.05),凝血酶原时间(PT)、凝血酶时间(TT)以及活化的部分凝血活酶时间(APTT)被延长,明显缩短优球蛋白溶解时间(ELT)的作用(P<0.05)。结论:红花通过C2-13菌种发酵炮制后可有效提升纤溶活性、抗凝作用,增大溶血栓的药效。

Objective： To explore the efficacy of bacillus fermentation processing Chinese medicine Flos Carthami in enhancing thrombolytic effect.Methods： Numerical system and system of numerical control technology were used for reasonable proper optimization of fermentation processing conditions.The carageen glue method was carried out on mice tail thrombosis experiment to test the fibrinolytic and blood coagulation index of rats.Results： Under the same dose（ 1 000 U/m L）,A red hair group（ Flos Carthami and C2-13 fermentation group） significantly shorten thrombus length than the rest of the groups（ P〈0.05）.The effective extension of thrombin time（ TT）,prothrombin time（ PT） and activated clotting time（ APTT） live enzymes were delayed,and significantly shorten euglobulin dissolution time（ ELT） effect（ P〈0.05）.Conclusion： Flos Carthami by C2-13 strains fermentation processing after anticoagulant effect,can effectively improve fibrinolytic activity,and increase the efficacy of dissolving thrombus.