对香豆酸对小鼠急性缺氧性肺水肿的预防作用研究

Effects of p-Coumaric Acid on Preventing Acute Hypoxia-Induced Pulmonary Edema Mice

目的采用动物试验研究对香豆酸(p-coumaric acid,p-CA)对急性缺氧性肺水肿的预防作用。方法建立急性缺氧小鼠模型,以红景天苷为阳性对照,连续灌胃给药7 d,通过检测急性缺氧处理后小鼠肺组织的含水量、炎症因子、抗氧化酶活性和Na^+,K^+-ATP酶活力,观察苏木素伊红(hematoxylin-eosin,HE)染色切片结果,对p-CA预防急性缺氧性肺水肿的效果及其可能的作用机制进行研究。结果与正常组相比,急性缺氧(9.5%O_2)6 h使小鼠肺组织含水量显著增加了3.56%(P<0.01),而25和100 mg·kg^(-1)·d^(-1)剂量的p-CA均能有效抑制其升高趋势(P<0.05),与红景天苷的效果相当。其作用机制可能与p-CA提高小鼠肺组织Na^+,K^+-ATP酶活力与抗氧化能力[超氧化物歧化酶(SOD)升高、过氧化氢酶(CAT)升高和丙二醛(MDA)下降]及降低炎症因子[白细胞介素(IL)-1β和IL-6]水平有关。结论p-CA对急性缺氧导致的肺水肿具有较好的预防作用。

OBJECTIVE To study the preventing effects of p-coumaric acid（p-CA） on acute hypoxia-induced pulmonary edema by mice experiments. METHODS Acute-hypoxia model was established using a normobaric hypoxia chamber in vivo. Salidroside was set as a positive control drug. And the test period was 7 d using a method of intragastric administration. The measurements including pulmonary water content, HE staining, inflammatory factors, anti-oxidative indexes and Na＋ , K ＋ -ATPase were performed to determine the efficacies and mechanisms of p-CA on preventive against acute hypoxia-induced pulmonary edema. RESULTS As compared with the normal group, pulmonary water contents increased significantly by 3.56% in the mice treated with acute hypoxia （9.5% 02） for 6 h （ control group） （ P 〈 0.01 ）, and administration with p-CA （25, 100 mg· kg-1 d-l ） for 7 d could significantly reduce this index （P 〈 0.05） , which was as effective as the positive group. The action mecha- nisms of p-CA could be due to its abilities of improving the activity of Na ＋ , K ＋-ATPase, enhancing antioxidant capacity （SOD ↑ , CAT ↑ and MDA ↓, ） and inhibiting inflammatory factors （ IL-1 β and IL-6）. CONCLUSION p-CA has greater preventive effects on acute hypoxia-induced pulmonary edema in mice.